Figure. Histologic changes in the spleen of sick Chinese giant salamanders (Andrias davidianus), People’s Republic of China, 2010. Electron microscopy shows virus particles in splenocytes. A) Hyperplasia of lymphoid nodules in the splenic white pulp. Inset: Some splenocytes contain nuclear debris (arrows) and macrophages (asterisk). Hematoxylin and eosin stain; scale bars = 80 μm. B) Electron microscopy image of viral particles in splenocytes. Many viral particles are cytoplasmic and appear hexagonal or round. Scale bar = 200 nm. VP, viral particles in cytoplasm; Nu, nucleus; arrowhead, provirus in nuclear membrane; arrows, provirus in nucleus.