Volume 17, Number 12—December 2011
Letter
Q Fever in Woolsorters, Belgium
Pierre Wattiau
, Eva Boldisova, Rudolf Toman, Marjan Van Esbroeck, Sophie Quoilin, Samia Hammadi, Hervé Tissot-Dupont, Didier Raoult, Jean-Marie Henkinbrant, Mieke Van Hessche, and David Fretin
, Eva Boldisova, Rudolf Toman, Marjan Van Esbroeck, Sophie Quoilin, Samia Hammadi, Hervé Tissot-Dupont, Didier Raoult, Jean-Marie Henkinbrant, Mieke Van Hessche, and David FretinTable A1
Coxiella burnetii TaqVet quantitative real-time PCR kit results, Belgium, 2007–2009*
| Material processed | Origin | Batch no. | Result | Ct | C. burnetii concentration, GenEq/mg |
|---|---|---|---|---|---|
| Goat hair | Afghanistan and Russia | 07/13528 1.1A | + | 30.0 | 103–104 |
| Goat hair | Afghanistan and Russia | 07/13528 1.1B | + | 29.0 | 103104 |
| Goat hair | Afghanistan and Russia | 07/13528 1.1C | + | 28.5 | 103–104 |
| Goat hair | Afghanistan | 06/11664.5 | + | 27.0 | 103–104 |
| Goat hair | Asia | 08/703.4 | + | 27.0 | 103–104 |
| Goat hair | Asia | 08/703.4 4B | + | 28.5 | 103–104 |
| Goat hair | Asia | 08/703.4 4C | + | 27.0 | 103–104 |
| Wool | The Netherlands | 33945 | + | 34.0 | <102 |
| Wool | France and the Netherlands | 33961 | + | 34.0 | <102 |
| Wool | Italy | 33944 | + | 36.0 | <102 |
| Wool | Hungary | 33954 | – | >40 | <10 |
| Wool | France and Switzerland | 33935 | + | 36.0 | <102 |
*DNA was extracted by using the QIAamp DNA Stool Mini Kit (QIAGEN, Valencia, CA, USA) from 20-mg of airborne dust samples suspended in 0.5 mL of MiliQ water (Millipore, Billerica, MA, USA). PCR (Laboratoire Service International, Lissieu, France) was conducted according to the manufacturer’s instructions. Ct, cycle threshold; GenEq, genome equivalents; +, positive; –, negative.
