Volume 17, Number 12—December 2011
Research
Experimental Infection of Horses with Hendra Virus/Australia/Horse/2008/Redlands
Glenn A. Marsh
, Jessica Haining, Timothy J. Hancock, Rachel Robinson, Adam Foord, Jennifer A. Barr, Shane Riddell, Hans G. Heine, John R. White, Gary Crameri, Hume E. Field, Lin-Fa Wang, and Deborah Middleton
, Jessica Haining, Timothy J. Hancock, Rachel Robinson, Adam Foord, Jennifer A. Barr, Shane Riddell, Hans G. Heine, John R. White, Gary Crameri, Hume E. Field, Lin-Fa Wang, and Deborah Middleton
Table
Real-time PCR detection of HeV in daily shedding samples in experimental infection of horses, Australia, 2008*
| Animal/sample | Cycle threshold values from HeV P gene real-time RT-PCR† |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Days postinfection |
||||||||||
| 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | |
| Horse 1 | ||||||||||
| Blood | − | − | − | − | 39.4 | 33.0 | 31.2 | |||
| Urine | − | − | − | 41.9 | − | 41.6 | 36.2 | |||
| Feces | − | − | − | − | − | 40.7 | 36.1 | |||
| Rectal swab | − | − | − | − | − | − | 42.0 | |||
| Nasal swab | − | − | 37.5 | 34.7 | 35.9 | 29.5 | 32.8 | |||
| Oral swab | − | − | − | − | − | 41.2 | 38.5 | |||
| Horse 2 | ||||||||||
| Blood | − | − | − | − | − | − | 37.3 | 32.2 | 31.4 | 29.9 |
| Urine | − | − | − | − | − | − | − | 36.2 | 36.3 | 33.5 |
| Feces | − | − | − | − | − | − | 40.7 | 37.0 | 35.0 | 35.2 |
| Rectal swab | − | − | − | − | − | − | − | 43.6 | 36.4 | 36.5 |
| Nasal swab | − | − | 36.3 | 32.4 | 38.9 | 34.3 | 31.1 | 28.1 | 29.2 | 35.2 |
| Oral swab | − | − | − | − | − | − | − | 36.6 | 35.8 | 34.5 |
| Horse 3 | ||||||||||
| Blood | − | − | − | − | − | 39.1 | 36.3 | 32.8 | 31.6 | |
| Urine | − | − | − | − | − | − | 40.7 | 39.0 | 34.3 | |
| Feces | − | − | − | − | 42.2 | − | 39.2 | 35.3 | 34.5 | |
| Rectal swab | − | − | − | − | − | − | − | 42.9 | 42.8 | |
| Nasal swab | − | − | 42.0 | NA | 41.4 | 43.0 | − | 37.9 | 32.9 | |
| Oral swab | − | − | − | − | 41.4 | 41.8 | 40.3 | 39.5 | 38.5 | |
*HeV, Hendra virus; RT-PCR, reverse transcription PCR; −, negative by real-time PCR testing; NA, sample not available for testing. Shading indicates that horse was euthanized.
†Individual samples were taken, RNA extracted, and samples tested by real-time PCR in duplicate.
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