Skip directly to search Skip directly to A to Z list Skip directly to site content
CDC Home

Volume 17, Number 12—December 2011

Research

Experimental Infection of Horses with Hendra Virus/Australia/Horse/2008/Redlands

Glenn A. MarshComments to Author , Jessica Haining, Timothy J. Hancock, Rachel Robinson, Adam Foord, Jennifer A. Barr, Shane Riddell, Hans G. Heine, John R. White, Gary Crameri, Hume E. Field, Lin-Fa Wang, and Deborah Middleton
Author affiliations: Commonwealth Scientific and Industrial Research Organization Livestock Industries, Geelong, Victoria, Australia (G.A. Marsh, J. Haining, T.J. Hancock, R. Robinson, A.J. Foord, J.A. Barr, S. Riddell, H.G. Heine, J.R. White, G. Crameri, L.-F. Wang, D. Middleton); Queensland Centre for Emerging Infectious Disease, Coopers Plains, Queensland, Australia (H.E. Field)

Main Article

Table

Real-time PCR detection of HeV in daily shedding samples in experimental infection of horses, Australia, 2008*

Animal/sample Cycle threshold values from HeV P gene real-time RT-PCR†
Days postinfection
0 1 2 3 4 5 6 7 8 9
Horse 1
Blood 39.4 33.0 31.2
Urine 41.9 41.6 36.2
Feces 40.7 36.1
Rectal swab 42.0
Nasal swab 37.5 34.7 35.9 29.5 32.8
Oral swab 41.2 38.5
Horse 2
Blood 37.3 32.2 31.4 29.9
Urine 36.2 36.3 33.5
Feces 40.7 37.0 35.0 35.2
Rectal swab 43.6 36.4 36.5
Nasal swab 36.3 32.4 38.9 34.3 31.1 28.1 29.2 35.2
Oral swab 36.6 35.8 34.5
Horse 3
Blood 39.1 36.3 32.8 31.6
Urine 40.7 39.0 34.3
Feces 42.2 39.2 35.3 34.5
Rectal swab 42.9 42.8
Nasal swab 42.0 NA 41.4 43.0 37.9 32.9
Oral swab 41.4 41.8 40.3 39.5 38.5

*HeV, Hendra virus; RT-PCR, reverse transcription PCR; −, negative by real-time PCR testing; NA, sample not available for testing. Shading indicates that horse was euthanized.
†Individual samples were taken, RNA extracted, and samples tested by real-time PCR in duplicate.

Main Article

Top of Page

USA.gov: The U.S. Government's Official Web PortalDepartment of Health and Human Services
Centers for Disease Control and Prevention   1600 Clifton Rd. Atlanta, GA 30333, USA
800-CDC-INFO (800-232-4636) TTY: (888) 232-6348 - Contact CDC–INFO