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Volume 17, Number 4—April 2011

Dispatch

Molecular Discrimination of Sheep Bovine Spongiform Encephalopathy from Scrapie

Laura Pirisinu, Sergio Migliore, Michele Angelo Di Bari, Elena Esposito, Thierry Baron, Claudia D’Agostino, Luigi De Grossi, Gabriele Vaccari, Umberto Agrimi, and Romolo NonnoComments to Author 
Author affiliations: Author affiliations: Istituto Superiore di Sanità, Rome, Italy (L. Pirisinu, S. Migliore, M.A. Di Bari, E. Esposito, C. D’Agostino, G. Vaccari, U. Agrimi, R. Nonno); Agence Nationale de Sécurité Sanitaire, Lyon, France (T. Baron); Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Rome (L. De Grossi)

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Table 1

Transmissible spongiform encephalopathy isolates analyzed by conformational stability and solubility assay*

Source Identification no. PrP genotype† GdnHCl1/2, mol/L ± SD‡
Natural isolates
Scrapie ES/8/10/2 ARQ/ARQ 2.19 ± 0.18
CH1641-like 99–454 VRQ/VRQ 2.00 ± 0.06
99–321 VRQ/VRQ 2.41 ± 0.49

TR316211
ARQ/ARQ
2.82 ± 0.08
Experimental samples
CH1641 241/74 AxQ/AxQ 2.07 ± 0.05
Sheep BSE 301/16§ ARQ/ARQ >4
301/44§ ARQ/ARQ >4
302/90¶ ARQ/ARQ 3.8; >4; >4

*PrP, prion protein; GdnHCL1/2, guanidine hydrochloride at a concentration able to dissolve half the insoluble aggregates in a brain homogenate; BSE, bovine spongiform encephalopathy.
†Amino acids at codons 136, 154 and 171.
‡Each sample was analyzed >3 times.
§Intracerebral transmission.
¶Oral transmission.

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