Volume 17, Number 4—April 2011
Dispatch
Rapid Genotyping of Swine Influenza Viruses
Polly W.Y. Mak, Chloe K.S. Wong, Olive T.W. Li, Kwok Hung Chan, Chung Lam Cheung, Edward S. Ma, Richard J. Webby, Yi Guan, Joseph S. Malik Peiris, and Leo L.M. Poon
Table 2
Summary of expected genotyping results of swine and human influenza viruses*
| Virus | PB2 | PB1 | PA | HA | NP | NA† | M | NS |
|---|---|---|---|---|---|---|---|---|
| Pandemic (H1N1) 2009 | + + | + + | + + | + + | + + | + + | + + | + + |
| Swine Eurasian avian-like | – – | – – | – – | – – | – – | – + | – + | – – |
| Swine triple reassortant | – + | – + | – + | – + | – + | – – | – – | – + |
| Human seasonal subtypes H1 and H3 | – – | – – | – – | – – | – – | – – | – – | – – |
*PB, polymerase basic protein; PA, polymerase acidic protein; HA, hemagglutinin; NP, nucleocapsid protein; NA, neuraminidase; M, matrix protein; NS, nonstructural protein. Red symbols indicate pandemic (H1N1) 2009 virus–specific probe results; black symbols indicate SYBR Green results; gray shading indicates sister clade of pandemic (H1N1) 2009 virus for each virus segment.
†N2 and some of the N1 within swine Euarasian avian-like lineage are expected to be double negative in the NA test.
