Volume 17, Number 5—May 2011
Experimental Infection of Amblyomma aureolatum Ticks with Rickettsia rickettsii
|Tick stage and generation||Infested hosts
||PCR of unfed ticks, no. infected/no. tested|
|Host, no.||No. ticks/host||Fever†
||No. deaths||Diagnostic test
|Nymph–F1||Guinea pig, 2||50||2||6||2||Pos||ND||45/45¶|
|Adult–F1||Dog, 2||20 couples||0||–||0||ND||ND||10/10|
|Larva–F2||Guinea pig, 2||1,000–2,000||2||7–8||1||ND||Pos||–|
|Nymph–F2||Guinea pig, 3||80||3||5||2||Pos||Pos||30/30|
|Adult–F2||Rabbit, 2||7 couples||2||7–8||0||ND||Pos||–|
|Larva–-F3||Guinea pig, 4||1,000–2,000||4||7–8||2||Pos||Pos||–|
|Adult–F3||Dog, 1||12 couples||0||–||0||ND||ND||–|
|Larva–F4||Guinea pig, 5||1,000–3,000||5||6–7||1||ND||Pos||100/100|
*dpi, days postinfestation; IFA, immunofluorescence assay; pos, positive; ND, not done; –, data not collected.
†Rectal temperature >40°C.
‡PCR performed on lung-extracted DNA from guinea pigs that died during the febrile period.
§A positive result means seroconversion, namely nonreactive (titer <64) at 0 DPI, and reactive (titer >1,024) at 21 dpi.
Previously reported by Labruna et al. (9).
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