Volume 17, Number 8—August 2011
Research
Novel Surveillance Network for Norovirus Gastroenteritis Outbreaks, United States1
Everardo Vega, Leslie Barclay, Nicole Gregoricus, Kara Williams, David Lee, and Jan Vinjé
Table 2
Oligonucleotide primers and probes used for detection and genotype identification of norovirus strains submitted to CaliciNet*
| Primer or probe name |
RT-PCR target |
Sequence, 5′ → 3′ |
Reference |
|---|---|---|---|
| TVN-L1 | ORF2–ORF3 | GGG TGT GTT GTG GTG TTG T26VN | (29) |
| L1 | ORF2–ORF3 | GGG TGT GTT GTG GTG TTG | This study |
| EVP2F | P2 (GII.4 specific) | GTR CCR CCH ACA GTT GAR TCA | This study |
| EVP2R | P2 (GII.4 specific) | CCG GGC ATA GTR GAY CTR AAG AA | This study |
| Cap D1 | Region D GII | TGT CTR STC CCC CAG GAA TG | (26) |
| Cap C | Region D GII | CCT TYC CAK WTC CCA YGG | (26) |
| Cap D3 | Region D GII | TGY CTY ITI CCH CAR GAA TGG | (26) |
| Cog 2F | ORF1–ORF2 junction (GII) | CAR GAR BCN ATG TTY AGR TGG ATG AG | (27) |
| Cog 2R | ORF1–ORF2 junction (GII) | TCG ACG CCA TCT TCA TTC ACA | (27) |
| Ring 2 | ORF1–ORF2 junction (GII) | Cy5-TGG GAG GGC GAT CGC AAT CT-BHQ | (27) |
| Ring 1C | ORF1–ORF2 junction (GI) | FAM-AGA TYG CGI TCI CCT GTC CA-BHQ | (28) |
| Cog 1F | ORF1–ORF2 junction (GI) | CGY TGG ATG CGI TTY CAT GA | (27) |
| Cog 1R | ORF1–ORF2 junction (GI) | CTT AGA CGC CAT CAT CAT TYA C | (27) |
*RT-PCR, reverse transcription PCR; ORF, open reading frame.
1Additional members of the CaliciNet participating laboratories who contributed data are listed at the end of this article.
