Volume 17, Number 8—August 2011
Bagaza Virus in Partridges and Pheasants, Spain, 2010
|Species||No. animals analyzed||Sample type||No. positive/no. tested
||Virus isolation in ECE, no. samples positive/no. tested†|
|A. rufa||11||Brain||0/11||11/11||1/3 (AF)|
|Kidney‡||0/1||1/1||2/3 (AF, CM, and VS)|
*West Nile virus (WNV) or Bagaza virus (BAGV) nucleic acids were identified by using real-time reverse transcription PCR (RT-PCR) or panflaviviral heminested RT-PCR, respectively, and amplicon (214 bp) sequencing. ECE, embryonated chicken egg; AF, allantoic fluid; NA, not analyzed; CM, chorioallantoic fluid; VS, viscera.
†ECEs were infected with homogenates of various samples.
‡Samples from which full-length sequence of BAGV was obtained.
§Tail, breast, and wing feathers from 1 partridge were analyzed.