Crimean-Congo Hemorrhagic Fever Virus in Ticks from Imported Livestock, Egypt
Katherine Chisholm, Erica Dueger, Nermeen T. Fahmy, Hamed Abdel Tawab Samaha, Alia Zayed, Mahmoud Abdel-Dayem, and Jeffrey T. Villinski
Author affiliations: US Naval Medical Research Unit No. 3, Cairo, Egypt (K. Chisholm, E. Dueger, N.T. Fahmy, A. Zayed, M. Abdel-Dayem, J.T. Villinski); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (E. Dueger); Ministry of Agriculture, Cairo (H.A.T. Samaha)
Figure. Phylogenetic relationship of 110 Crimean-Congo hemorrhagic fever virus sequences obtained from this study (6 tick pools), the positive control, and 99 published isolates from GenBank. Sequences were aligned by using ClustalX (www.clustal.org), and a phylogenetic tree was constructed by using MEGA4 (neighbor-joining, Kimura 2-parameter, complete deletion of missing data, tree condensed when bootstrap calculated branch support was <0.5) (www.megasoftware.net). The 2 shaded areas indicate clustering of African isolates (AFR) and predominantly European isolates (EUR); unshaded isolates originate primarily from the Middle East.
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