Emerging Infectious Disease ISSN: 1080-6059

Constant Transmission Properties of Variant Creutzfeldt-Jakob Disease in 5 Countries

Abigail B. Diack, Diane Ritchie, Matthew Bishop, Victoria Pinion, Jean-Philippe Brandel, Stephane Haik, Fabrizio Tagliavini, Cornelia Van Duijn, Ermias D. Belay, Pierluigi Gambetti, Lawrence B. Schonberger, Pedro Piccardo, Robert G. Will¹, and Jean C. Manson¹

Author affiliations: The Roslin Institute, Easter Bush, Scotland, UK (A.B. Diack, V. Pinion, J.C. Manson); University of Edinburgh, Edinburgh, Scotland, UK (D. Ritchie, M. Bishop, R.G. Will); Cellule Nationale de Référence des Maladies de Creutzfeldt-Jakob, Universite Pierre et Marie Curie-Paris 6, INSERM, and CNRS, Paris, France (J.-P. Brandel, S. Haik); Fdazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy (F. Tagliavini); Erasmus University Medical School, Rotterdam, the Netherlands (C. Van Duijn); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (E.D. Belay, L.B. Schonberger); Case Western Reserve University, Cleveland, Ohio, USA (P. Gambetti); and Food and Drug Administration, Rockville, Maryland, USA (P. Piccardo)

Main Article

Figure 4

Western blot analysis of brain extracts from RIII (A) and VM (B) wild-type mice inoculated with variant Creutzfeldt-Jakob disease (vCJD) brain tissue. Lane 1, human vCJD brain homogenate (UK origin) showing the typical abnormal prion protein (PrPSc) type 2B; lane 2, United Kingdom; lane 3, The Netherlands; lane 4: Italy (cortex); lane 5, Italy (cerebellum); lane 6, France; lane 7, United States; lane 8, human sporadic Creutzfeldt-Jakob disease brain homogenate showing the typical PrPSc type 1. T

Figure 4. . Western blot analysis of brain extracts from RIII (A) and VM (B) wild-type mice inoculated with variant Creutzfeldt-Jakob disease (vCJD) brain tissue. Lane M, positive control; lane 1, human vCJD brain homogenate (UK origin) showing the typical abnormal prion protein (PrP^Sc) type 2B; lane 2, United Kingdom; lane 3, The Netherlands; lane 4: Italy (cortex); lane 5, Italy (cerebellum); lane 6, France; lane 7, United States; lane 8, human sporadic Creutzfeldt-Jakob disease brain homogenate showing the typical PrP^Sc type 1. Type 2B and 1 differ in mobility of the unglycosylated band (≈19 kDa and ≈20 kDa, respectively). All samples were treated with proteinase K. The anti–prion protein detection antibody used was 6H4.

Main Article

¹Joint senior authors.

Top of Page

The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.