Figure. . Maximum-likelihood tree of an ≈200-bp fragment of the nonstructural 5 gene of a reverse transcription PCR–positive West Nile virus (WNV) specimen SAH5238/08 (GenBank accession no. JX974605; black diamond) isolated from a human in South Africa in 2008. The tree shows the relationship of the strain to representative sequences of 5 WNV lineages, including 5 WNV lineage 2 strains isolated from horses in South Africa in 2008 (15). The scale bar indicates nucleotide substitutions per site. Bootstrap statistics of >70% are indicated on the tree branches. WNV strains (accession numbers): B956 (AY532665), SA381/00 (EF429199), SA93/01 (EF429198), SPU116/89 (EF429197), Goshawk-Hungary/04 (DQ116961), H442 (EF429200), Sarafend (AY688948), Madagascar AnMg798 (DQ176636), HS123/08 (FJ464376), HS101/08 (FJ464378), SAE126/08 (FJ464379), SAE134/08 (FJ464380), HS125/08 (FJ464377), Rabensburg97103 (AY765264), LEIV-Krnd88-190 (AY277251), Kunjin (D00246), Egypt101 (AF260968), EthAn4766 (AY603654), Italy1998 (AF404757), Goose-Hungary/03 (DQ118127), NY385-99 (EF571854), TX2002 (DQ164205), Mexico2003 (AY660002), IND804994 (DQ256376), Japanese encephalitis ( HM228921).
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