Volume 18, Number 12—December 2012
Research
Reservoir Competence of Wildlife Host Species for Babesia microti
Michelle H. Hersh
, Michael Tibbetts, Mia Strauss, Richard S. Ostfeld, and Felicia Keesing
, Michael Tibbetts, Mia Strauss, Richard S. Ostfeld, and Felicia KeesingTable 3
Sequences of Babesia microti 18S rDNA from newly molted nymphal Ixodes scapularis ticks fed on vertebrate hosts aligned with sequences from known zoonotic isolates and raccoon isolates from GenBank, southeastern New York, USA, 2008–2010*
| Species | Sequence, 5′ → 3′ |
|---|---|
| Didelphis virginiana A20030_21 | AAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGTATTCAACGAGTTTTTTCCTTGGC |
| Procyon lotor 506_10 | AAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGTATTCAACGAGTTTTTTCCTTGGC |
| Babesia microti raccoon isolate, USA (AY144701) | AAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGTATTCAACGAGTTTTTTCCTTGGC |
| B. microti raccoon isolate, Japan (AB197940) | AAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGTATTCAACGAGTTTTTTCCTTGGC |
| Blarina brevicauda BB3_10 | AAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGCATTCAACGAG-TTTTTCCTTGGC |
| Catharus fuscescens, 2341–01870_17 | AAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGCATTCAACGAG-TTTTTCCTTGGC |
| Hylocichla mustelina 1951–10576_14 | AAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGCATTCAACGAG-TTTTTCCTTGGC |
| Peromyscus leucopus A20003_10 | AAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGCATTCAACGAG-TTTTTCCTTGGC |
| Sciurus carolinensis E412_14 | AAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGCATTCAACGAG-TTTTTCCTTGGC |
| Tamias striatus H1037_11 | AAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGCATTCAACGAG-TTTTTCCTTGGC |
| B. microti GI strain (AF231348) | AAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGCATTCAACGAG-TTTTTCCTTGGC |
| B. microti Jena/Germany isolate (EF413181) | AAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGCATTCAACGAG-TTTTTCCTTGGC |
| B. microti Kobe isolate (AB032434) | AAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGCATTCAACGAG--TTTTCCTTGGC |
*Sequences for zoonotic isolates were obtained from Gray et al. (4). The 85–86-bp sequence is the region of 18S rDNA between primers smbaJF and smbaKR. Consistent 2-bp differences between ticks collected from different host species are indicated in boldface. The empty row separates sequences with basepair differences. Accessions nos. for GenBank sequences are indicated in parentheses after isolate names. GI, American isolate.
