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Volume 18, Number 12—December 2012

Research

Nonprimate Hepaciviruses in Domestic Horses, United Kingdom

Sinéad Lyons, Amit Kapoor, Colin Sharp, Bradley S. Schneider, Nathan D. Wolfe, Geoff Culshaw, Brendan Corcoran, Bruce C. McGorum, and Peter SimmondsComments to Author 
Author affiliations: Author affiliations: University of Edinburgh, Edinburgh, Scotland, UK (S. Lyons, C. Sharp, G. Culshaw, B. Corcoran, B.C. McGorum, P. Simmonds); Columbia University, New York, New York, USA (A. Kapoor); Global Viral Forecasting Initiative, San Francisco, California, USA (B.S. Schneider, N.D. Wolfe)

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Table 2

Nonprimate hepaciviruses primer sequences for 5′UTR, NS3, and NS5B in samples from horses, United Kingdom*

Primer
Position
Sequence, 5′ →3′
EQ5→UTROS Forward outer sense ACA YYA CCA TGT GTC ACT CCC CCT
EQ5→UTROAS Reverse outer antisense CYC ATG TCC TAT GGT CTA CGA GA
EQ5→UTRIS Forward inner sense ACA CGG AAA YGG GTT AAC CAY ACY C
EQ5→UTRIAS Reverse inner antisense GCC CTC GCA AGC ATC CTA TCA G
EQNS3OS Forward outer sense ATW TGT GAT GAR TGC CAY AGY AC
EQNS3OAS Reverse outer antisense TAG TAG GTB ACA GCR TTA GCY CC
EQNS3IS Forward inner sense TCY AAR GGT GTD AAG CTT GTT GT
EQNS3IAS Reverse inner antisense TGG CAG AAG YTA AGR TGY CTY CC
EQNS5BIS Forward outer sense AAR TGY TTT GAC TCY ACB GTC ACT C
EQNS5BOIAS Reverse outer antisense ACT RTG ACT RAT YGT YTC CCA ACT CG
EQNS5BIS2 Forward inner sense CAY GAT ATA GAH ACT GAG AGR GA
EQNS5BIAS2 Reverse inner antisense TCR TCT TCC TCR ACG CCY TTR CTG G

*UTR, untranslated region; NS, nonstructural protein.

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