Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 18, Number 12—December 2012
Dispatch

No Evidence of Prolonged Hendra Virus Shedding by 2 Patients, Australia

Carmel Taylor, Elliott G. Playford, William J.H. McBride, Jamie McMahon, and David WarrilowComments to Author 
Author affiliations: Queensland Health Forensic and Scientific Services, Archerfield, Queensland, Australia (C. Taylor, J. McMahon, D. Warrilow); Princess Alexandra Hospital, Brisbane, Queensland, Australia (E.G. Playford); University of Queensland School of Medicine, Brisbane (E.G. Playford); and James Cook University School of Medicine and Dentistry, Cairns, Queensland, Australia (W.J.H. McBride)

Main Article

Figure

Serologic and quantitative reverse transcription PCR (qRT-PCR) results for samples from 2 patients infected with Hendra virus, Australia. Testing was performed from the time at which symptoms first developed (black vertical line) until the most recent sample indicated (dashed vertical arrows). IgG (black bars) and IgM (white bars) reactivity was determined by using a modified microsphere immunoassay (10), and a positive control serum sample to determine the cutoff value. Virus RNA was detected (

Figure. . Serologic and quantitative reverse transcription PCR (qRT-PCR) results for samples from 2 patients infected with Hendra virus, Australia. Testing was performed from the time at which symptoms first developed (black vertical line) until the most recent sample indicated (dashed vertical arrows). IgG (black bars) and IgM (white bars) reactivity was determined by using a modified microsphere immunoassay (10), and a positive control serum sample to determine the cutoff value. Virus RNA was detected (gray box) or not detected (black box or triangles) by using a qRT-PCR (11). The horizontal arrow indicates 3 samples stored at −80°C and tested retrospectively for Hendra virus RNA. Positive and negative controls were included in all tests and showed expected results.

Main Article

References
  1. Field  HE, Mackenzie  JS, Daszak  P. Henipaviruses: emerging paramyxoviruses associated with fruit bats. Curr Top Microbiol Immunol. 2007;315:133–59. DOIPubMedGoogle Scholar
  2. Goh  KJ, Tan  CT, Chew  NK, Tan  PS, Kamarulzaman  A, Sarji  SA, Clinical features of Nipah virus encephalitis among pig farmers in Malaysia. N Engl J Med. 2000;342:1229–35. DOIPubMedGoogle Scholar
  3. Tan  CT, Goh  KJ, Wong  KT, Sarji  SA, Chua  KB, Chew  NK, Relapsed and late-onset Nipah encephalitis. Ann Neurol. 2002;51:703–8. DOIPubMedGoogle Scholar
  4. Chong  HT, Chong  TT. Relapsed and late-onset Nipah encephalitis, a report of three cases. Neurology Journal of Southeast Asia. 2003;8:109–12.
  5. O’Sullivan  JD, Allworth  AM, Paterson  DL, Snow  TM, Boots  R, Gleeson  LJ, Fatal encephalitis due to novel paramyxovirus transmitted from horses. Lancet. 1997;349:93–5. DOIPubMedGoogle Scholar
  6. Wong  KT, Robertson  T, Ong  BB, Chong  JW, Yaiw  KC, Wang  LF, Human Hendra virus infection causes acute and relapsing encephalitis. Neuropathol Appl Neurobiol. 2009;35:296–305. DOIPubMedGoogle Scholar
  7. Australian veterinary emergency plan. Version 3.3; 2009 [cited 2012 Jun 28]. http://www.animalhealthaustralia.com.au/wp-content/uploads/2011/04/RPB3.3-05FINAL27Jul11.pdf
  8. Hanna  JN, McBride  WJ, Brookes  DL, Shield  J, Taylor  CT, Smith  IL, Hendra virus infection in a veterinarian. Med J Aust. 2006;185:562–4.PubMedGoogle Scholar
  9. Playford  EG, McCall  B, Smith  G, Slinko  V, Allen  G, Smith  I, Human Hendra virus encephalitis associated with equine outbreak, Australia, 2008. Emerg Infect Dis. 2010;16:219–23. DOIPubMedGoogle Scholar
  10. Bossart  KN, McEachern  JA, Hickey  AC, Choudhry  V, Dimitrov  DS, Eaton  BT, Neutralization assays for differential henipavirus serology using Biol-Plex protein array systems. J Virol Methods. 2007;142:29–40. DOIPubMedGoogle Scholar
  11. Smith  IL, Halpin  K, Warrilow  D, Smith  GA. Development of a fluorogenic RT-PCR assay (TaqMan) for the detection of Hendra virus. J Virol Methods. 2001;98:33–40. DOIPubMedGoogle Scholar
  12. Davis  LE, DeBiasi  R, Goade  DE, Haaland  KY, Harrington  JA, Harnar  JB, West Nile virus neuroinvasive disease. Ann Neurol. 2006;60:286–300. DOIPubMedGoogle Scholar
  13. Kapoor  H, Signs  K, Somsel  P, Downes  FP, Clark  PA, Massey  JP. Persistence of West Nile virus (WNV) IgM antibodies in cerebrospinal fluid from patients with CNS disease. J Clin Virol. 2004;31:289–91. DOIPubMedGoogle Scholar
  14. Garg  RK. Subacute sclerosing panencephalitis. J Neurol. 2008;255:1861–71. DOIPubMedGoogle Scholar

Main Article

Page created: November 21, 2012
Page updated: November 21, 2012
Page reviewed: November 21, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external