Emerging Infectious Disease ISSN: 1080-6059

No Evidence of Prolonged Hendra Virus Shedding by 2 Patients, Australia

Carmel Taylor, Elliott G. Playford, William J.H. McBride, Jamie McMahon, and David Warrilow

Author affiliations: Queensland Health Forensic and Scientific Services, Archerfield, Queensland, Australia (C. Taylor, J. McMahon, D. Warrilow); Princess Alexandra Hospital, Brisbane, Queensland, Australia (E.G. Playford); University of Queensland School of Medicine, Brisbane (E.G. Playford); and James Cook University School of Medicine and Dentistry, Cairns, Queensland, Australia (W.J.H. McBride)

Main Article

Figure

Serologic and quantitative reverse transcription PCR (qRT-PCR) results for samples from 2 patients infected with Hendra virus, Australia. Testing was performed from the time at which symptoms first developed (black vertical line) until the most recent sample indicated (dashed vertical arrows). IgG (black bars) and IgM (white bars) reactivity was determined by using a modified microsphere immunoassay (10), and a positive control serum sample to determine the cutoff value. Virus RNA was detected (

Figure. . Serologic and quantitative reverse transcription PCR (qRT-PCR) results for samples from 2 patients infected with Hendra virus, Australia. Testing was performed from the time at which symptoms first developed (black vertical line) until the most recent sample indicated (dashed vertical arrows). IgG (black bars) and IgM (white bars) reactivity was determined by using a modified microsphere immunoassay (10), and a positive control serum sample to determine the cutoff value. Virus RNA was detected (gray box) or not detected (black box or triangles) by using a qRT-PCR (11). The horizontal arrow indicates 3 samples stored at −80°C and tested retrospectively for Hendra virus RNA. Positive and negative controls were included in all tests and showed expected results.

Main Article

Top of Page

The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.