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Volume 18, Number 12—December 2012

Dispatch

No Evidence of Prolonged Hendra Virus Shedding by 2 Patients, Australia

Carmel Taylor, Elliott G. Playford, William J.H. McBride, Jamie McMahon, and David WarrilowComments to Author 
Author affiliations: Queensland Health Forensic and Scientific Services, Archerfield, Queensland, Australia (C. Taylor, J. McMahon, D. Warrilow); Princess Alexandra Hospital, Brisbane, Queensland, Australia (E.G. Playford); University of Queensland School of Medicine, Brisbane (E.G. Playford); and James Cook University School of Medicine and Dentistry, Cairns, Queensland, Australia (W.J.H. McBride)

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Figure

Serologic and quantitative reverse transcription PCR (qRT-PCR) results for samples from 2 patients infected with Hendra virus, Australia. Testing was performed from the time at which symptoms first developed (black vertical line) until the most recent sample indicated (dashed vertical arrows). IgG (black bars) and IgM (white bars) reactivity was determined by using a modified microsphere immunoassay (10), and a positive control serum sample to determine the cutoff value. Virus RNA was detected (

Figure. . Serologic and quantitative reverse transcription PCR (qRT-PCR) results for samples from 2 patients infected with Hendra virus, Australia. Testing was performed from the time at which symptoms first developed (black vertical line) until the most recent sample indicated (dashed vertical arrows). IgG (black bars) and IgM (white bars) reactivity was determined by using a modified microsphere immunoassay (10), and a positive control serum sample to determine the cutoff value. Virus RNA was detected (gray box) or not detected (black box or triangles) by using a qRT-PCR (11). The horizontal arrow indicates 3 samples stored at −80°C and tested retrospectively for Hendra virus RNA. Positive and negative controls were included in all tests and showed expected results.

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