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Volume 18, Number 3—March 2012
Dispatch

Culturing Stool Specimens for Campylobacter spp., Pennsylvania, USA

Nkuchia M. M’ikanatha, Lisa A. Dettinger, Amanda Perry, Paul Rogers, Stanley M. Reynolds, and Irving NachamkinComments to Author 
Author affiliations: Pennsylvania Department of Health, Harrisburg, Pennsylvania, USA (N.M. M’ikanatha, A. Perry); Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA (N.M. M’ikanatha, I. Nachamkin); Pennsylvania Department of Health, Exton, Pennsylvania, USA (L.A. Dettinger, P. Rogers, S.M. Reynolds)

Main Article

Table 2

Campylobacter-specific laboratory practices for 107 Pennsylvania laboratories performing testing of stool specimens, 2009*

Laboratory practice/method No. (%) laboratories
Included in routine testing 104 (97.2)
Special request culture 1 (0.9)
Culture plus antigen testing 1 (0.9)
Perform culture on positive antigen assay 2 (1.9)
Campylobacter antigen testing only 17 (15.8)
Culture broth enrichment usage
Yes (9 Campy-Thio, 1 GNB, 4 unspecified) 14 (13.1)
No 89 (83.2)
No response 4 (3.7)
Length of Incubation, h
24 1 (<1)
48 64 (59.8)
72 33 (30.8)
No response 9 (8.4)
Type of medium used for Campylobacter culture
Campy-BAP 65 (60.7)
CVA 30 (27.8)
Skirrow 2 (1.9)
CSM 2 (1.8)
CCDA or mCCDA 0
Not specified or not cultured 8 (7.5)
Temperature used for culture, °C
37 3 (2.8)
42 96 (89.7)
Not specified 8 (7.5)
Atmosphere used for culture
Microaerobic, 5% O2 96 (89.7)
10% CO2 1 (0.9)
Both 1(0.9)
Not specified 9 (8.4)
Tests used for identification of Campylobacter spp.
Gram stain 96 (89.7)
Oxidase 92 (86)
Catalase 70 (65.4)
Hippurate hydrolysis 51 (47.7)
Naladixic acid/cephalothin disk identification 21 (19.6)
Indoxyl acetate 7 (6.5)
Send to a reference laboratory 6 (5.6)
Other, not specified 33 (30.8)
Performs susceptibility testing 4 (3.7)

*Campy-Thio, Campylobacter thioglycollate broth; GNB, gram-negative broth; Campy-BAP, Campylobacter blood agar plates; CVA, cefoperazone-vancomycin-amphotericin; CSM, charcoal-based selective medium; CCDA, charcoal-cefoperazone-deoxycholate agar; mCCDA, modified CCDA.

Main Article

Page created: February 16, 2012
Page updated: February 16, 2012
Page reviewed: February 16, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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