Volume 18, Number 4—April 2012
Research
Identification of Intermediate in Evolutionary Model of Enterohemorrhagic Escherichia coli O157
Table
Fifty strains used for SNP typing of enterohemorrhagic Escherichia coli O157*
| Strain ID | Year of isolation | Illness | SF status and serotype | Subgroup/cluster† | Reference and/or GenBank accession no. |
|---|---|---|---|---|---|
| TB182A‡ | 1991 | D | SF O55:H7 | A | (12) |
| CB9615‡ | 2003 | D | SF O55:H7 | A | NC_013941 |
| 493/89‡ | 1989 | HUS | SF O157:H– | B | (16) |
| 87–14‡ | 1987 | HUS | NSF O157:H7 | C1 | (12) |
| EC4115‡ | 2006 | BD | NSF O157:H7 | C1 | NC_011353 |
| TW14359‡ | 2006 | BD | NSF O157:H7 | C1 | NC_013008 (12,17) |
| TW14588‡ | 2006 | BD | NSF O157:H7 | C3 | NZ_ABKY00000000.2 |
| 86–24‡ | 1986 | HUS | NSF O157:H7 | C2 | (12) |
| Sakai‡ | 1996 | D | NSF O157:H7 | C3 | NC_002695 (18) |
| EDL933‡ | 1983 | NA | NSF O157:H7 | C3 | (14) |
| LSU-61 | 2001 | NA | SF O157:H7 | Unknown intermediate | (10,13) |
| SNPO157_01 | 1987 | HUS | NSF O157:H7 | C1 | This study |
| SNPO157_02 | 1988 | D | NSF O157:H7 | C1 | This study |
| SNPO157_03 | 1988 | HUS | SF O157:H– | B | This study |
| SNPO157_04 | 1990 | HUS | NSF O157:H7 | C1 | This study |
| SNPO157_05 | 1991 | HUS | NSF O157:H– | C3 | This study |
| SNPO157_06 | 1992 | HUS | NSF O157:H7 | C3 | This study |
| SNPO157_07 | 1993 | HUS | NSF O157:H7 | C1 | This study |
| SNPO157_08 | 1995 | HUS | NSF O157:H7 | C1 | This study |
| SNPO157_09 | 1995 | HUS | SF O157:H– | B | This study |
| SNPO157_10 | 1996 | HUS | NSF O157:H– | C1 | This study |
| SNPO157_11 | 1996 | HUS | SF O157:H– | B | This study |
| SNPO157_12 | 1996 | HUS | SF O157:H– | B | This study |
| SNPO157_13 | 1996 | HUS | NSF O157:H7 | C1 | This study |
| SNPO157_14 | 1997 | HUS | NSF O157:H7 | C1 | This study |
| SNPO157_15 | 1997 | HUS | NSF O157:H7 | C1 | This study |
| SNPO157_16 | 1998 | HUS | NSF O157:H7 | C1 | This study |
| SNPO157_17 | 1999 | HUS | NSF O157:H7 | C1 | This study |
| SNPO157_18 | 1999 | HUS | NSF O157:H7 | C3 | This study |
| SNPO157_19 | 2000 | HUS | NSF O157:H7 | C1 | This study |
| SNPO157_20 | 2000 | D | NSF O157:H7 | C3 | This study |
| SNPO157_21 | 2001 | HUS | SF O157:H– | B | This study |
| SNPO157_22 | 2001 | HUS | NSF O157:H– | C3 | This study |
| SNPO157_23 | 2002 | D | NSF O157:H7 | C3 | This study |
| SNPO157_24 | 2002 | A | NSF O157:H7 | C1 | This study |
| SNPO157_25 | 2003 | HUS | NSF O157:H7 | C3 | This study |
| SNPO157_26 | 2004 | HUS | NSF O157:H7 | C1 | This study |
| SNPO157_27 | 2005 | HUS | NSF O157:H7 | C3 | This study |
| SNPO157_28 | 2005 | BD | NSF O157:H7 | C3 | This study |
| SNPO157_29 | 2005 | HUS | NSF O157:H7 | C1 | This study |
| SNPO157_30 | 2006 | HUS | NSF O157:H7 | C3 | This study |
| SNPO157_31 | 2007 | HUS | NSF O157:H7 | C3 | This study |
| SNPO157_32 | 2007 | HUS | NSF O157:H7 | C3 | This study |
| SNPO157_33 | 2008 | HUS | NSF O157:H7 | C3 | This study |
| SNPO157_34 | 2008 | D | SF O157:H– | B | This study |
| SNPO157_35 | 2008 | HUS | SF O157:H– | B | This study |
| SNPO157_36 | 2009 | D | NSF O157:H7 | § | This study |
| SNPO157_37 | 2009 | HUS | SF O157:H– | B | This study |
| SNPO157_38 | 2010 | HUS | NSF O157:H7 | C3 | This study |
| SNPO157_39 | 2010 | HUS | SF O157:H– | B | This study |
*All strains were isolated from humans except strain LSU-61, which was isolated from a deer (10), and EDL933, which was isolated from food (14). Strains isolated from humans were categorized into 3 subgroups (11,12); subgroup A represents isolates of serotype O55:H7, subgroup B SF O157:H– isolates, and subgroup C NSF O157:H7. Subgroup C is subdivided into clusters 1–3. SNP, single-nucleotide polymorphism; ID, identification; SF, sorbitol fermenting; D, diarrhea; HUS, hemolytic uremic syndrome; NSF, non-SF; BD, bloody diarrhea; NA, not applicable; A, asymptomatic.
†Subgroup and, if applicable, cluster designation based on 4 SNP loci (Sakai genome positions 337933, 1460599, 2370797, and 5404166) and the occupancy of potential stx integration sites in accordance with (11,12,15). Boldface indicates cluster designation of prototype strains.
‡Strains were analyzed in silico.
§SNP pattern for NSF O157:H7 grouping resulted in an unknown combination.
