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Volume 18, Number 8—August 2012
Dispatch

Putative Novel Genotype of Avian Hepatitis E Virus, Hungary, 2010

Krisztián Bányai, Ádám György Tóth, Éva Ivanics, Róbert Glávits, Katalin Szentpáli-Gavallér, and Ádám DánComments to Author 
Author affiliations: Hungarian Academy of Sciences, Budapest, Hungary (K. Bányai); and Veterinary Diagnostic Directorate, Budapest (Á.G. Tóth, É. Ivanics, R. Glávits, K. Szentpáli-Gavallér, Á. Dán)

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Figure

Genomic structure of HUN-16773-2010 and 5 other avian hepatitis E virus (aHEV) strains. A) Neighbor-joining tree constructed from the nucleotide sequences (14) of the nearly complete genome of 6 aHEV strains. Bootstrap values are indicated at branch nodes. A swine HEV served as outgroup in the phylogeny. Genotype assignments are depicted for reference aHEV genomes. Scale bar indicates genetic distance. B) Adaptive evolution of aHEV genomes. Only sites under positive selection in open reading fra

Figure. . . . Genomic structure of HUN-16773-2010 and 5 other avian hepatitis E virus (aHEV) strains. A) Neighbor-joining tree constructed from the nucleotide sequences (14) of the nearly complete genome of 6 aHEV strains. Bootstrap values are indicated at branch nodes. A swine HEV served as outgroup in the phylogeny. Genotype assignments are depicted for reference aHEV genomes. Scale bar indicates genetic distance. B) Adaptive evolution of aHEV genomes. Only sites under positive selection in open reading frame 1 are shown with normalized value of dN – dS along the codon positions, as indicated by the random effects likelihood method and by using the default significance level (www.datamonkey.org).

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References
  1. Meng  XJ. Hepatitis E virus: animal reservoirs and zoonotic risk. Vet Microbiol. 2010;140:256–65. DOIPubMedGoogle Scholar
  2. Payne  CJ, Ellis  TM, Plant  SL, Gregory  AR, Wilcox  GE. Sequence data suggests big liver and spleen disease virus (BLSV) is genetically related to hepatitis E virus. Vet Microbiol. 1999;68:119–25. DOIPubMedGoogle Scholar
  3. Haqshenas  G, Shivaprasad  HL, Woolcock  PR, Read  DH, Meng  XJ. Genetic identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis-splenomegaly syndrome in the United States. J Gen Virol. 2001;82:2449–62.PubMedGoogle Scholar
  4. Morrow  CJ, Samu  G, Mátrai  E, Klausz  A, Wood  AM, Richter  S, Avian hepatitis E virus infection and possible associated clinical disease in broiler breeder flocks in Hungary. Avian Pathol. 2008;37:527–35. DOIPubMedGoogle Scholar
  5. Ivanics  É, Benyeda  Z, Benyeda  J, Nagy  G, Dán  Á, Tóth  Á, Examination of hepatitis-E virus induced disease in Hungarian laying hen flocks [in Hungarian]. Magy Allatorv Lapja. 2011;133:587–93.
  6. Bilic  I, Jaskulska  B, Basic  A, Morrow  CJ, Hess  M. Sequence analysis and comparison of avian hepatitis E viruses from Australia and Europe indicate the existence of different genotypes. J Gen Virol. 2009;90:863–73. DOIPubMedGoogle Scholar
  7. Johne  R, Plenge-Bönig  A, Hess  M, Ulrich  RG, Reetz  J, Schielke  A. Detection of a novel hepatitis E–like virus in faeces of wild rats using a nested broad-spectrum RT-PCR. J Gen Virol. 2010;91:750–8. DOIPubMedGoogle Scholar
  8. Billam  P, Sun  ZF, Meng  XJ. Analysis of the complete genomic sequence of an apparently avirulent strain of avian hepatitis E virus (avian HEV) identified major genetic differences compared with the prototype pathogenic strain of avian HEV. J Gen Virol. 2007;88:1538–44. DOIPubMedGoogle Scholar
  9. Huang  FF, Sun  ZF, Emerson  SU, Purcell  RH, Shivaprasad  HL, Pierson  FW, Determination and analysis of the complete genomic sequence of avian hepatitis E virus (avian HEV) and attempts to infect rhesus monkeys with avian HEV. J Gen Virol. 2004;85:1609–18. DOIPubMedGoogle Scholar
  10. Zhao  Q, Zhou  EM, Dong  SW, Qiu  HK, Zhang  L, Hu  SB, Analysis of avian hepatitis E virus from chickens, China. Emerg Infect Dis. 2010;16:1469–72. DOIPubMedGoogle Scholar
  11. Marek  A, Bilic  I, Prokofieva  I, Hess  M. Phylogenetic analysis of avian hepatitis E virus samples from European and Australian chicken flocks supports the existence of a different genus within the Hepeviridae comprising at least three different genotypes. Vet Microbiol. 2010;145:54–61. DOIPubMedGoogle Scholar
  12. Koonin  EV, Gorbalenya  AE, Purdy  MA, Rozanov  MN, Reyes  GR, Bradley  DW. Computer-assisted assignment of functional domains in the nonstructural polyprotein of hepatitis E virus: delineation of an additional group of positive-strand RNA plant and animal viruses. Proc Natl Acad Sci U S A. 1992;89:8259–63. DOIPubMedGoogle Scholar
  13. Martin  DP, Lemey  P, Lott  M, Moulton  V, Posada  D, Lefeuvre  P. RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics. 2010;26:2462–3. DOIPubMedGoogle Scholar
  14. Tamura  K, Peterson  D, Peterson  N, Stecher  G, Nei  M, Kumar  S. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28:2731–9. DOIPubMedGoogle Scholar
  15. Pudupakam  RS, Kenney  SP, Córdoba  L, Huang  YW, Dryman  BA, Leroith  T, Mutational analysis of the hypervariable region of hepatitis E virus reveals its involvement in the efficiency of viral RNA replication. J Virol. 2011;85:10031–40. DOIPubMedGoogle Scholar

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