Volume 18, Number 8—August 2012
Dispatch
Putative Novel Genotype of Avian Hepatitis E Virus, Hungary, 2010
Krisztián Bányai, Ádám György Tóth, Éva Ivanics, Róbert Glávits, Katalin Szentpáli-Gavallér, and Ádám Dán
Table
Primers used in the generation of nearly complete genome of the novel Hungarian avian hepatitis E virus strain*
| Primer name | Sequence, 5′ → 3′ | Location | Orientation |
|---|---|---|---|
| F1 | CAG ACT ACC ATT ATC GCC AC | 2926–2945 | + |
| R1 | AGC TGG AGC TTG ACA ACT TC | 3319–3338 | – |
| R1b | AGC TGG AGC TTG ACA ACC TC | 3319–3338 | – |
| F2 | GGA TGC CTG ACT GGA TGG T | 3926–3944 | + |
| F2b | CTG ACT GGA TGG TGG CTC T | 3932–3950 | + |
| R2 | TTA CCA GGC GAC AGA TTC C | 5395–5413 | – |
| F3 | ACT CGT GTT AAG GTA ACG GC | 5430–5449 | + |
| F3b | GTG GCT GAA GAT GGC GAG AT | 5541–5560 | + |
| F3c | GGT GTG GCT GAA GAT GGC GA | 5538–5557 | + |
| R3 | CCG AGA TGG GAG GAT TTC ATA A | 6629–6650† | – |
| F4 | GCA GTT TGC AGA GTC CAA GG | 36–55† | + |
| R4 | CTG ATC AAA AGT GCT ACC CT | 2909–2928 | – |
| R4b | TGA TTG ACC CGG GGT TCG | 2876–2893 | – |
| F5 | GGT GTT GAG GTC GGC ATG GT | 4432–4451 | + |
| F5b | ATA TCC AGC GCA TGC AGG | 4325–4342 | + |
| R5 | ATC TAC ATC TGG TAC CGT GC | 4828–4847 | – |
| R5b | GAC GTT GTG CCA TCC TGA AG | 4965–4984 | – |
| F6 | TCC TGT TCT CAC CAG TGT | 400–417 | + |
| R6 | AGC CCA TAA TTA ACC ATG TC | 2302–2321 | – |
| R6b | GAT GTC GAT TTA CCG CTG CC | 2413–2432 | – |
| F7 | GGT GTG GCA GAG GTT AAT GAT G | 897–918 | + |
| F7b | GAT GAT GCC TTC TGC TGC TC | 937–956 | + |
| R7 | AAG ACA GCA AGG ACC TCC TC | 1795–1814 | – |
| R7b | AGG CTG ATG ATC ACG GTT GG | 1883–1902 | – |
*The annealing temperature was set at 53°C for each primer combination. +, sense; –, antisense.
†5′ and 3′ end primers were designed on the basis of sequence AM943646.
