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Volume 18, Number 8—August 2012

Dispatch

Putative Novel Genotype of Avian Hepatitis E Virus, Hungary, 2010

Krisztián Bányai, Ádám György Tóth, Éva Ivanics, Róbert Glávits, Katalin Szentpáli-Gavallér, and Ádám DánComments to Author 
Author affiliations: Hungarian Academy of Sciences, Budapest, Hungary (K. Bányai); and Veterinary Diagnostic Directorate, Budapest (Á.G. Tóth, É. Ivanics, R. Glávits, K. Szentpáli-Gavallér, Á. Dán)

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Table

Primers used in the generation of nearly complete genome of the novel Hungarian avian hepatitis E virus strain*

Primer name Sequence, 5′ → 3′ Location Orientation
F1 CAG ACT ACC ATT ATC GCC AC 2926–2945 +
R1 AGC TGG AGC TTG ACA ACT TC 3319–3338
R1b AGC TGG AGC TTG ACA ACC TC 3319–3338
F2 GGA TGC CTG ACT GGA TGG T 3926–3944 +
F2b CTG ACT GGA TGG TGG CTC T 3932–3950 +
R2 TTA CCA GGC GAC AGA TTC C 5395–5413
F3 ACT CGT GTT AAG GTA ACG GC 5430–5449 +
F3b GTG GCT GAA GAT GGC GAG AT 5541–5560 +
F3c GGT GTG GCT GAA GAT GGC GA 5538–5557 +
R3 CCG AGA TGG GAG GAT TTC ATA A 6629–6650†
F4 GCA GTT TGC AGA GTC CAA GG 36–55† +
R4 CTG ATC AAA AGT GCT ACC CT 2909–2928
R4b TGA TTG ACC CGG GGT TCG 2876–2893
F5 GGT GTT GAG GTC GGC ATG GT 4432–4451 +
F5b ATA TCC AGC GCA TGC AGG 4325–4342 +
R5 ATC TAC ATC TGG TAC CGT GC 4828–4847
R5b GAC GTT GTG CCA TCC TGA AG 4965–4984
F6 TCC TGT TCT CAC CAG TGT 400–417 +
R6 AGC CCA TAA TTA ACC ATG TC 2302–2321
R6b GAT GTC GAT TTA CCG CTG CC 2413–2432
F7 GGT GTG GCA GAG GTT AAT GAT G 897–918 +
F7b GAT GAT GCC TTC TGC TGC TC 937–956 +
R7 AAG ACA GCA AGG ACC TCC TC 1795–1814
R7b AGG CTG ATG ATC ACG GTT GG 1883–1902

*The annealing temperature was set at 53°C for each primer combination. +, sense; –, antisense.
†5′ and 3′ end primers were designed on the basis of sequence AM943646.

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