Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
Chao Qiu1, Yang Huang1, Anli Zhang1, Di Tian1, Yanmin Wan1, Xiaoling Zhang, Wanju Zhang, Zhiyong Zhang, Zhenghong Yuan, Yunwen Hu, Xiaoyan Zhang, and Jianqing Xu
Author affiliations: Shanghai Public Health Clinical Center, Shanghai, China (C. Qiu, A. Zhang, D. Tian, Y. Wan, Xiaoling Zhang, W. Zhang, Z. Zhang, Z. Yuan, Y. Hu, Xiaoyan Zhang, J. Xu); Fudan University, Shanghai (C. Qiu, Y. Huang, A. Zhang, Z. Yuan, Xiaoyan Zhang, J. Xu); Chinese Center for Disease Control and Prevention, Beijing, China (Xiaoyan Zhang, J. Xu)
Figure. . Correlation between conventional hemagluttination (HI) titer and 50% inhibitory concentration (IC50) titer of pseudovirus-based assay for diagnosing influenza A(H7N9) virus infection. Correlation between the IC50 titer of the pseudovirus-based neutralization assay and the titer of conventional HI assay, tested with 14 serum samples collected >10 days after symptom onset from patients with real-time RT-PCR–confirmed 2013 influenza A(H7N9) infection (○) and 50 control samples (△, Spearman r = 0.88, p<0.0001, n = 64). To display the information from all the samples, overlapped markers were shifted on the x- and/or y-axis with small incremental units.
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.