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Volume 19, Number 2—February 2013
Dispatch

Genetic Variants of Echovirus 13, Northern India, 2010

Harjeet Singh Maan, Rashmi Chowdhary, Akhalesh Kumar Shakya, and Tapan N. DholeComments to Author 
Author affiliations: Author affiliations: Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India (H.S. Maan, A.K. Shakya, T.N. Dhole); Columbia University, New York, New York, USA (R. Chowdhary)

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Figure

Phylogenetic tree based on alignments of partial viral protein 1 gene sequences of echovirus 13 (EV13) constructed by the neighbor-joining method implemented in MEGA version 5.05 software (7) by using the Kimura-2 parameter nucleotide substitution model. Bootstrap analysis included 1,000 pseudoreplicate datasets. Clusters are labeled A, B, C, and D. Square indicates Uttar Pradesh EV13 from fecal samples. All Uttar Pradesh EV13 isolates on the tree are identified by using the same numbers listed

Figure. . Phylogenetic tree based on alignments of partial viral protein 1 gene sequences of echovirus 13 (E13) constructed by the neighbor-joining method implemented in MEGA version 5.05 software (7) by using the Kimura-2 parameter nucleotide substitution model. Bootstrap analysis included 1,000 pseudoreplicate datasets. Clusters are labeled A, B, C, and D. Square indicates Uttar Pradesh E13 from fecal samples. All Uttar Pradesh E13 isolates on the tree are identified by using the same numbers listed in Table 1. Triangle indicates E13 prototype Del Carmen and the out-group enterovirus 69. Scale bar indicates nucleotide substitutions per site.

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References
  1. Oberste  MS, Maher  K, Flemister  MR, Marchetti  G, Kilpatrick  DR, Pallansch  MA. Comparison of classic and molecular approaches for the identification of untypeable enteroviruses. J Clin Microbiol. 2000;38:11704 .PubMedGoogle Scholar
  2. Oberste  MS, Maher  K, Kilpatrick  DR, Pallansch  MA. Molecular evolution of the human enteroviruses: correlation of serotype with VP1 sequence and application to picornavirus classification. J Virol. 1999;73:19418 .PubMedGoogle Scholar
  3. Dhole  TN, Ayyagari  A, Chowdhary  R, Shakya  A, Shrivastav  N, Datta  T, Non-polio enteroviruses in acute flaccid paralysis children of India: vital assessment before polio eradication. J Paediatr Child Health. 2009;45:40913. DOIPubMedGoogle Scholar
  4. World Health Organization. Polio laboratory manual. 4th ed. Geneva: The Organization; 2004.
  5. Dias  AP, Tavares  FN, Costa  EV, da Silva  EE. Evaluation of a protocol for rapid diagnosis of enterovirus associated with acute flaccid paralysis cases. J Clin Virol. 2009;46:33740. DOIPubMedGoogle Scholar
  6. Oberste  MS, Nix  WA, Maher  K, Pallansch  MA. Improved molecular identification of enteroviruses by RT-PCR and amplicon sequencing. J Clin Virol. 2003;26:3757. DOIPubMedGoogle Scholar
  7. Tamura  K, Peterson  D, Peterson  N, Stecher  G, Nei  M, Kumar  S. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28:27319. DOIPubMedGoogle Scholar
  8. Junttila  N, Leveque  N, Kabue  JP, Cartet  G, Mushiya  F, Muyembe-Tamfum  JJ, New enteroviruses, EV-93 and EV-94, associated with acute flaccid paralysis in the Democratic Republic of the Congo. J Med Virol. 2007;79:393400. DOIPubMedGoogle Scholar
  9. Pérez-Vélez  CM, Anderson  MS, Robinson  CC, McFarland  EJ, Nix  WA, Pallansch  MA, Outbreak of neurologic enterovirus type 71 disease: a diagnostic challenge. Clin Infect Dis. 2007;45:9507. DOIPubMedGoogle Scholar
  10. Khetsuriani  N, Kutateladze  T, Zangaladze  E, Shutkova  T, Penaranda  S, Nix  WA, High degree of genetic diversity of non-polio enteroviruses identified in Georgia by environmental and clinical surveillance, 2002–2005. J Med Microbiol. 2010;59:13407. DOIPubMedGoogle Scholar
  11. Kumar  A, Shukla  D, Kumar  R, Idris  MZ, Misra  UK, Dhole  TN. An epidemic of encephalitis associated with human enterovirus B in Uttar Pradesh, India, 2008. J Clin Virol. 2011;51:1425. DOIPubMedGoogle Scholar
  12. Kumar  A, Shukla  D, Kumar  R, Idris  MZ, Jauhari  P, Srivastava  S, Molecular identification of enteroviruses associated with aseptic meningitis in children from India. Arch Virol. 2012; Epub ahead of print. DOIPubMedGoogle Scholar
  13. Kobayashi  K, Haruta  T, Kubota  M, Akiyoshi  K, Suga  T, Ito  M. Clinical spectrum in hospitalized children with echovirus type 13 infection. Pediatr Int. 2005;47:1859. DOIPubMedGoogle Scholar
  14. Apostol  LN, Suzuki  A, Bautista  A, Galang  H, Paladin  FJ, Fuji  N, Detection of non-polio enteroviruses from 17 years of virological surveillance of acute flaccid paralysis in the Philippines. J Med Virol. 2012;84:62431. DOIPubMedGoogle Scholar
  15. Mullins  JA, Khetsuriani  N, Nix  WA, Oberste  MS, LaMonte  A, Kilpatrick  DR, Emergence of echovirus type 13 as a prominent enterovirus. Clin Infect Dis. 2004;38:707. DOIPubMedGoogle Scholar

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