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Volume 19, Number 2—February 2013

Letter

Coxiella burnetii in Ticks, Argentina

Richard C. Pacheco, Ignacio E. Echaide, Rosiane N. Alves, Marcelo E. Beletti, Santiago Nava, and Marcelo B. LabrunaComments to Author 
Author affiliations: Author affiliations: Federal University of Mato Grosso, Cuiabá, Brazil (R.C. Pacheco); Instituto Nacional de Tecnología Agropecuaria, Rafaela, Argentina (I.E. Echaide, S. Nava); Federal University of Uberlândia, Uberlândia, Brazil (R.N. Alves, M.E. Beletti); University of São Paulo, São Paulo, Brazil (M.B. Labruna)

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Figure

Vero cells inoculated with Amblyomma tick extracts for isolation of rickettsiae. A) Rickettsiae-like organisms stained in red by Gimenez staining (original magnification ×400). B) Inoculated monolayer photographed under phase-contrast microscopy (original magnification ×400). C) Uninfected control monolayer under phase-contrast microscopy (original magnification ×400). D) Transmission electron microscopy of infected cells. E) Transmission electron microscopy image of intravacuolar bacteria. Bar

Figure. . Vero cells inoculated with Amblyomma tick extracts for isolation of rickettsiae. A) Rickettsiae-like organisms stained in red by Gimenez staining (original magnification ×400). B) Inoculated monolayer photographed under phase-contrast microscopy (original magnification ×400). C) Uninfected control monolayer under phase-contrast microscopy (original magnification ×400). D) Transmission electron microscopy of infected cells. Bar indicates 2 µm. N, nucleolus. E) Transmission electron microscopy image of intravacuolar bacteria. Bar indicates 250 nm. Arrows indicate vacuoles containing bacteria.

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