Richard C. Pacheco, Ignacio E. Echaide, Rosiane N. Alves, Marcelo E. Beletti, Santiago Nava, and Marcelo B. Labruna
Author affiliations: Author affiliations: Federal University of Mato Grosso, Cuiabá, Brazil (R.C. Pacheco); Instituto Nacional de Tecnología Agropecuaria, Rafaela, Argentina (I.E. Echaide, S. Nava); Federal University of Uberlândia, Uberlândia, Brazil (R.N. Alves, M.E. Beletti); University of São Paulo, São Paulo, Brazil (M.B. Labruna)
Figure. . Vero cells inoculated with Amblyomma tick extracts for isolation of rickettsiae. A) Rickettsiae-like organisms stained in red by Gimenez staining (original magnification ×400). B) Inoculated monolayer photographed under phase-contrast microscopy (original magnification ×400). C) Uninfected control monolayer under phase-contrast microscopy (original magnification ×400). D) Transmission electron microscopy of infected cells. Bar indicates 2 µm. N, nucleolus. E) Transmission electron microscopy image of intravacuolar bacteria. Bar indicates 250 nm. Arrows indicate vacuoles containing bacteria.
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