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Volume 19, Number 2—February 2013

Dispatch

Usutu Virus, Italy, 1996

Herbert WeissenböckComments to Author , Tamás Bakonyi, Giacomo Rossi, Paolo Mani, and Norbert Nowotny
Author affiliations: Author affiliations: University of Veterinary Medicine, Vienna, Austria (H. Weissenböck, T. Bakonyi, N. Nowotny); Szent István University, Budapest, Hungary (T. Bakonyi); University of Camerino, Matelica, Italy (G. Rossi); University of Pisa, Pisa, Italy (P. Mani); Sultan Qaboos University, Muscat, Oman (N. Nowotny)

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Table

Results of IHC analysis and real-time RT-PCR for Usutu virus in birds, Italy, 1996*

Group, protocol no.
Tissue
Bird species
IHC result
RT-PCR result
1
6439-A1 Lung, kidney, spleen Turdus merula + (kidney) +
6439-C2 Brain T. merula + +
6439-C3 Brain T. merula + +
6439-C4 Brain T. merula + +
6439-D5 Proventriculus, gizzard T. merula +
6439-D6 Kidney T. merula +
6439-D7
Liver
T. merula
I
+
2
6484-A Skeletal muscle T. merula
6484-B Spleen T. merula
6484-C Intestine T. merula
6484-D Liver T. merula
6484-E Heart T. merula
6484-F
Lung
T. merula
I

3
7665-A1 Brain T. merula, T. iliacus, Sturnus vulgaris
7665-A2 Brain T. merula, T. iliacus, S. vulgaris
7665-B1 Brain T. merula, T. iliacus, S. vulgaris
7665-B2 Brain, intestine T. merula, T. iliacus, S. vulgaris
7665-B3 Liver, lung, kidney T. merula, T. iliacus, S. vulgaris
7665-C1 Brain T. merula, T. iliacus, S. vulgaris
7665-C2 Heart T. merula, T. iliacus, S. vulgaris
7665-C3 Liver, intestine, skeletal muscle T. merula, T. iliacus, S. vulgaris
7665-D1 Brain T. merula, T. iliacus, S. vulgaris
7665-D2
Liver, kidney
T. merula, T. iliacus, S. vulgaris


4
7714–1 Heart T. merula, T. pilaris
7714–2 Liver, kidney T. merula, T. pilaris
7714–3 Spleen, bursa T. merula, T. pilaris
7714–4 Brain T. merula, T. pilaris
7714–5 Brain T. merula, T. pilaris
7714–6 Intestine, pancreas T. merula, T. pilaris
7714–7 Heart T. merula, T. pilaris
7714–8 Heart T. merula, T. pilaris
7714–9 Brain T. merula, T. pilaris
7714–10 Kidney T. merula, T. pilaris

*IHC, immunohistochemical; RT-PCR, reverse transcription PCR; +, positive; –, negative; I, inconclusive.
†Blocks not assignable to individual bird species.

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