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Volume 19, Number 6—June 2013

Synopsis

New Delhi Metallo-β-Lactamase–producing Enterobacteriaceae, United States

J. Kamile RasheedComments to Author , Brandon Kitchel, Wenming Zhu, Karen F. Anderson, Nancye C. Clark, Mary Jane Ferraro, Patrice Savard, Romney M. Humphries, Alexander J. Kallen, and Brandi M. Limbago
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J.K. Rasheed, B. Kitchel, W. Zhu, K.F. Anderson, N.C. Clark, A.J. Kallen, B.M. Limbago); Massachusetts General Hospital, Boston, Massachusetts, USA (M.J. Ferraro); Johns Hopkins University, Baltimore, Maryland, USA (P. Savard); Johns Hopkins Health System, Baltimore (P. Savard); University of California Los Angeles David Geffen School of Medicine, Los Angeles, California, USA (R.M. Humphries)

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Figure 1

XmnI restriction analysis of New Delhi metallo-β-lactamase (NDM)–encoding plasmids, United States, April 2009–March 2011, from transformants (A) and subsequent Southern blot analysis with digoxigenin-labeled blaNDM probe hybridized to a blot of same gel (B). Lane 1, NDM PCR product, positive control; lane 2, NDM-negative plasmid (ATCC-1705); lanes 3 and 14, 1-kb plus marker; lane 4, TF 0S-506; lane 5, TF 1100770; lane 6, TF 1100975; lane 7, TF1100192; lane 8, TF 1000527; lane 9, TF 1101459; lane

Figure 1. . XmnI restriction analysis of New Delhi metallo-β-lactamase (NDM)–encoding plasmids, United States, April 2009–March 2011, from transformants (A) and subsequent Southern blot analysis with digoxigenin-labeled blaNDM probe hybridized to a blot of same gel (B). Lane 1, NDM PCR product, positive control; lane 2, NDM-negative plasmid (ATCC-1705); lanes 3 and 14, 1-kb plus marker; lane 4, TF 0S-506; lane 5, TF 1100770; lane 6, TF 1100975; lane 7, TF1100192; lane 8, TF 1000527; lane 9, TF 1101459; lane 10, TF 1101168; lane 11, TF 1100101; lane 12, TF 1001728; lane 13, TF 1000654.

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