Volume 2, Number 3—July 1996
Synopsis
Application of Molecular Techniques to the Diagnosis of Microsporidial Infection
Daniel P. Fedorko
and Yasmine M. Hijazi
and Yasmine M. HijaziTable 2
SSU-rRNA PCR primer pairs for the diagnosis of microsporidial infection
| Primer pair (no. nucleotides) | Primer designation | Organisms amplified | Amplicon size in base pairs | Source of Target | References | |
|---|---|---|---|---|---|---|
| 5'-CACCAGGTTGATTCTGCCTGAC-3' | (22) | V1 | Enterocytozoon bieneusi | 348 | Biopsied tissue Duodenal aspirates | 33, 41 31 |
| 5'-ACTCAGGTGTTATACTCACGTC-3' | (22) | EB450 | Encephalitozoon hellem | Cultured organisms | 33 | |
| 5'-GAAACTTGTCCACTCCTTACG-3' | (21) | EBIEF1 | E.bieneusi | 607 | Bile | 43 |
| 5'-CCATGCACCACTCCTGCCATT-3' | (21) | EBIER1 | Duodenal aspirates | |||
| 5'-TGAGAAGTAAGATGTTTAGCA-3' | (21) | E.hellem | 547 | Cultured organisms | 39 | |
| 5'-GTAAAAACACTCTCACACTCA-3' | (21) | |||||
| 5'-ATGAGAAGTGATGTGTGTGCG-3' | (21) | Encephalitozoon cuniculi | 549 | Cultured organisms | 39, 40 | |
| 5'-TGCCATGCACTCACAGGCATC-3' | (21) | |||||
| 5'-CACCAGGTTGATTCTGCCTGAC-3' | (22) | V1 | Encephalitozoon intestinalis | 370 | Biopsied tissue | 31 |
| 5'-CTCGCTCCTTTACACTCGAA-3' | (20) | SI500 | ||||
| 5'-CACCAGGTTGATTCTGCCTGAC-3' | (22) | PMP1* | E.bieneusi | 250 | Stool | 42 |
| 5'-CCTCTCCGGAACCAAACCTG-3' | (21) | PMP2 | E.cuniculli | 268 | Cultured organisms | |
| E.intestinalis | 270 | Stool | ||||
| E.hellem | 279 | Cultured organisms | ||||
*The nucleotide sequence of primer PMP1 is identical to that of primer V1.
