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Volume 20, Number 2—February 2014

Research

Human Antibody Responses to Avian Influenza A(H7N9) Virus, 2013

Li Guo1, Xi Zhang1, Lili Ren1, Xuelian Yu1, Lijuan Chen1, Hongli Zhou, Xin Gao, Zheng Teng, Jianguo Li, Jiayu Hu, Chao Wu, Xia Xiao, Yiyi Zhu, Quanyi Wang, Xinghuo Pang, Qi Jin2, Fan Wu2, and Jianwei Wang2Comments to Author 
Author affiliations: MOH Key Laboratory of Systems Biology of Pathogens, Beijing, People’s Republic of China (L. Guo, L. Ren, J. Li, Q. Jin, J. Wang); Institute of Pathogen Biology, Beijing (L. Guo, L. Ren, H. Zhou, X. Gao, J. Li, C. Wu, X. Xiao, Q. Jin, J. Wang); Shanghai Municipal Center for Disease Control and Prevention, Shanghai, People’s Republic of China (X. Zhang, X. Yu, Z. Teng, J. Hu, Y. Zhu, F. Wu); Beijing Municipal Center for Disease Control and Prevention, Beijing (L. Chen, Q. Wang, X. Pang)

Main Article

Figure 1

Serum antibodies (Abs) in patients infected with influenza A(H7N9) virus and in control populations (poultry-market workers and healthy blood donors), China, 2013. A) Dilution curves of IgG against subtype H7 in serum samples. Bars indicate SE. B and C) Titers of IgG against H7, H1, and H3 in poultry-market workers (B) and healthy blood donors (C). D) Increasing titers of IgG against subtype H7 after symptom onset in patients from whom paired serum samples were collected. E) Levels of IgG agains

Figure 1. Serum antibodies (Abs) in patients infected with influenza A(H7N9) virus and in control populations (poultry-market workers and healthy blood donors), China, 2013A) Dilution curves of IgG against subtype H7 in serum samplesBars indicate SEB and C) Titers of IgG against H7, H1, and H3 in poultry-market workers (B) and healthy blood donors (C)D) Increasing titers of IgG against subtype H7 after symptom onset in patients from whom paired serum samples were collectedE) Levels of IgG against H7, neutralizing antibodies (NAbs), and hemagglutination inhibition (HI) in serum samples after symptom onsetIgG against hemagglutinins of H7 and seasonal influenza A viruses (subtypes H1 and H3) in patients with subtype H7N9 virus infection and control populations were titrated by ELISA by using recombinant hemagglutinin antigensNAbs were assessed by microneutralization assay the influenza A/Anhui/1/2013 (H7N9) strainHI antibodies (Abs) were assessed by HI assay that used a β-propriolactone–inactivated influenza A/Anhui/1/2013 (H7N9) strainB–E) Serum IgG, NAb, and HI titers were transformed to log10For NAb-negative samples, titers of 2 were used for log10 transformationSerum with titers >40 were considered HI positive for H7-specific antibodyThe HI dotted line denotes a titer of log1040 = 1.60Serum with titers >20 were considered NAb positive for H7-specific antibodyThe NAb dotted line denotes a titer of log1020 = 1.30*, not available.

Main Article

1These authors contributed equally to this article and are co–first authors.

2These authors contributed equally to this article and are co–senior authors.

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