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Volume 20, Number 8—August 2014


Novel Reassortant Influenza A(H5N8) Viruses in Domestic Ducks, Eastern China

Haibo Wu, Xiaorong Peng, Lihua Xu, Changzhong Jin, Linfang Cheng, Xiangyun Lu, Tiansheng Xie, Hangping Yao, and Nanping WuComments to Author 
Author affiliations: Zhejiang University, Hangzhou, China (H. Wu, X. Peng, C. Jin, L. Cheng, X. Lu, T. Xie, H. Yao, N. Wu); Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou (H. Wu, X. Peng, C. Jin, L. Cheng, X. Lu, T. Xie, H. Yao, N. Wu); Zhejiang Academy of Agricultural Science, Hangzhou (L. Xu)

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Table 2

Characteristics of 2 novel influenza A(H5N8) viruses isolated from domestic ducks, eastern China, 2013 *

Virus Characteristic
Virus replication in experimentally infected mice, virus titers in organs of mice (log10 EID50/mL)†
IVPI EID50 TCID50 Tissue 3 d 6 d 9 d
W24 3.0 108.5 108.4 Lung 2.5 ± 0.5 3.0 ± 0.5 5.0 ± 0.5
NA NA NA Brain
NA NA NA Liver
6D18 3.0 108.5 109.6 Lung 2.0 ± 0.5 2.5 ± 0.5 3.0 ± 0.5
NA NA NA Brain
NA NA NA Heart
NA NA NA Liver 2.0 ± 0.5

*Groups of 14 adult female BALB/c mice were intranasally inoculated with a 106 median egg infective dose (EID50) of each virus in a 0.05-mL volume of phosphate-buffered saline. Five mice per group were observed for survival within 14 d. The remaining 9 mice from each experimental group were exsanguinated on days 3, 6, and 9, and their lungs, brains, hearts and livers were collected for virus titration in embryonated chicken eggs. IVPI, intravenous pathogenicity index; TCID50, median tissue culture infective dose; NA, not applicable.
†Values are mean ± SD. –, virus was not detected.

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