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Volume 21, Number 1—January 2015
Dispatch

Rapid Diagnostic Tests for Identifying Avian Influenza A(H7N9) Virus in Clinical Samples

Yu Chen1, Dayan Wang1, Shufa Zheng, Junhua Li, Wenxiang Chen, Dawei Cui, Jinming Li, Weizhong Yang, Yu Wang, Lanjuan Li2, and Hong Shang2Comments to Author 
Author affiliations: Zhejiang University, Hangzhou, China (Y. Chen, S. Zheng, D. Cui, L. Li); Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou (Y. Chen, S. Zheng, D. Cui, L. Li); National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China (D. Wang, Y. Shu); Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou (D. Wang, Y. Shu); Beijing Hospital Institute of Geriatrics and National Center for Clinical Laboratories, Beijing (W. Chen, J. Li); Chinese Center for Disease Control and Prevention, Beijing (H. Yu, Y. Wang); The First Hospital of China Medical University, Shenyang, China (H. Shang)

Main Article

Table 2

RDT positivity rates for detection of different influenza A virus subtypes in real-time reverse transcription PCR–positive specimens*

Ct
 Wantai Flu A Dot-ELISA† Wondfo Flu A test, colloidal gold method† Wondfo H7 Subtype test, colloidal gold method
H7N9
 H1N1 pdm09
 H3N2
H7N9
 H1N1 pdm09
 H3N2
H7N9
 H1N1 pdm09
 H3N2
<25 7/7 (100) 12/14 (86) 22/24 (92) 7/7 (100) 12/14 (86) 22/24 (92) 7/7 (100) 0/14 (0) 0/24 (0)
25–30 18/38 (47) 35/54 (65) 35/55 (64) 11/38 (29) 23/54 (43) 23/55 (42) 28/38 (74) 0/54 (0) 0/55 (0)
>30
 13/65 (20)
 8/29 (28)
 15/36 (42)
6/65 (9)
 7/29 (24)
 15/36 (42)
21/65 (32)
 0/29 (0)
 0/36 (0)
Total 38/110 (35) 55/97 (57) 72/115 (63) 24/110 (22) 42/97 (43) 60/115 (52) 56/110 (51) 0/97 (0) 0/115 (0)

*Values are no. specimens positive by RDT/no. specimens positive by real-time reverse transcription PCR (%). Ct , cycle threshold; RDT, rapid diagnostic test.
†Sensitivity for influenza A (H7N9) virus was significantly lower than that for either H1N1 pdm09 or influenza A (H3N2) viruses (p<0.01, χ2 test), but no statistically significant difference in sensitivity was found between A(H1N1)pdm09 and influenza A (H3N2) viruses.

Main Article

1These authors contributed equally to this article.

2These authors contributed equally to this article.

Page created: December 19, 2014
Page updated: December 19, 2014
Page reviewed: December 19, 2014
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