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Volume 21, Number 1—January 2015
Research

Protocol for Metagenomic Virus Detection in Clinical Specimens1

Claudia KohlComments to Author , Annika Brinkmann, Piotr W. Dabrowski, Aleksandar Radonić, Andreas Nitsche, and Andreas Kurth
Author affiliations: Robert Koch Institute, Berlin, Germany

Main Article

Figure 10

Changes in virus-to-host nucleic acid signal-to-noise ratio during development of tissue-based universal virus detection for viral metagenomics (TUViD-VM) protocol. Next-generation sequencing results for virus-infected marmoset tissue comparatively sequenced were obtained by using 4 approaches: standard RNA library preparation (Monkey RNA), standard DNA library preparation (Monkey DNA), DNA library from random-amplified marmoset tissue (Monkey random), and DNA library from random-amplified marmo

Figure 10. Changes in virus-to-host nucleic acid signal-to-noise ratio during development of tissue-based universal virus detection for viral metagenomics (TUViD-VM) protocol. Next-generation sequencing results for virus-infected marmoset tissue comparatively sequenced were obtained by using 4 approaches: standard RNA library preparation (Monkey RNA), standard DNA library preparation (Monkey DNA), DNA library from random-amplified marmoset tissue (Monkey random), and DNA library from random-amplified marmoset tissue prepared by using TUViD-VM (Monkey purified random). The y-axis is log-scaled.

Main Article

1Preliminary results of this study were presented at the Biodefense and Emerging Infectious Diseases Meeting, January 29, 2014, Washington DC, USA.

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