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Volume 21, Number 5—May 2015
Dispatch

Influenza A(H5N8) Virus Similar to Strain in Korea Causing Highly Pathogenic Avian Influenza in Germany

Timm HarderComments to Author , Sebastian Maurer-Stroh, Anne Pohlmann, Elke Starick, Detlef Höreth-Böntgen, Karin Albrecht, Gunter Pannwitz, Jens P. Teifke, Vithiagaran Gunalan, Raphael T.C. Lee, Carola Sauter-Louis, Timo Homeier, Christoph Staubach, Carola Wolf, Günter Strebelow, Dirk Höper, Christian Grund, Franz J. Conraths, Thomas C. Mettenleiter, and Martin Beer
Author affiliations: Friedrich-Loeffler-Institut, Insel Riems, Germany (T. Harder, A. Pohlmann, E. Starick, D. Höreth-Böntgen, J. Teifke, C. Sauter-Louis, T. Homeier, C. Staubach, G. Strebelow, D. Höper, C. Grund, F.J. Conraths, T.C. Mettenleiter, M. Beer); Nanyang Technological University, Singapore (S. Maurer-Stroh); Ministry of Health, Singapore (S. Maurer-Stroh); Agency for Science, Technology and Research, Singapore (S. Maurer-Stroh, V. Gunalan, R.T.C. Lee); County Veterinary Office, Anklam, Germany (K. Albrecht, G. Pannwitz); Agency for Agriculture, Food Security and Fisheries of the German Federal State of Mecklenburg–Western Pomeranio, Rostock, Germany (C. Wolf)

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Figure 2

Phylogenetic analysis of hemagglutinin (HA) 1 nucleotide sequences of highly pathogenic avian influenza viruses subtype H5 from Southeast Asia and Germany. Insert shows the structural model of the HA protein of the German H5N8 isolate AR2472/14. A) Nucleotide sequences encoding the membrane-distal part of the HA1 of influenza A(H5N8) viruses were retrieved from public databases, aligned by using MAFFT (http://mafft.cbrc.jp/alignment/software) and phylogenetically analyzed by using a maximum-like

Figure 2. Phylogenetic analysis of hemagglutinin (HA) 1 nucleotide sequences of highly pathogenic avian influenza viruses subtype H5 from Southeast Asia and Germany. Insert shows the structural model of the HA protein of the German H5N8 isolate AR2472/14. A) Nucleotide sequences encoding the membrane-distal part of the HA1 of influenza A(H5N8) viruses were retrieved from public databases, aligned by using MAFFT (http://mafft.cbrc.jp/alignment/software) and phylogenetically analyzed by using a maximum-likelihood approach (best fit model: K3Pu+G4) implemented in IQ-Tree (http://www.cibiv.at/software/iqtree) (12). Numbers at nodes represent surrogates of branching robustness obtained by an ultrafast bootstrap approach (12). Scale bar indicates nucleotide substitutions per site. B) Model of an HA monomer of AR2472/14 with PDB:3FKU used as template. Green depicts unique mutations distinguishing this virus from other South Korea–origin avian influenza (H5N8) viruses; red indicates additional substitutions relative to the closest vaccine candidate within clade 2.3.4.4. (A/Sichuan/26221/2014 [H5N6]).

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Page updated: April 18, 2015
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