Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 22, Number 10—October 2016
Letter

Unmet Needs for a Rapid Diagnosis of Chikungunya Virus Infection

Elisa BurdinoComments to Author , Guido Calleri, Pietro Caramello, and Valeria Ghisetti
Author affiliations: Amedeo di Savoia Hospital, Torino, Italy

Main Article

Table

Reported sensitivity and specificity of rapid point-of-care tests for detecting chikungunya virus, 2008–2015*

Reference and test(s) Time from symptom onset to testing, d Sensitivity, %‡ Specificity, %‡ Test reference standard
(1)
OnSite Chikungunya IgM Rapid Test 1 to >21 20.5 100 Capture ELISA IgM (in house) with Asian lineage virus; rRT-PCR
SD BIOLINE Chikungunya IgM test
 1 to >21
 50.8
 89.2
 Capture ELISA IgM (in house) with Asian lineage virus; rRT-PCR
(2)
SD BIOLINE Chikungunya IgM test
 <7; 8 to >14§
 22; 83
 88; 71
 ELISA IgM; rRT-PCR
(3)
OnSite Chikungunya IgM Rapid Test
 3.75 to >7
 12.1
 100
 IgM IFA; capture ELISA IgM (in house); rRT-PCR
(4)
SD BIOLINE Chikungunya IgM test
 3–8
 1.9–3.9
 92.5–95.0
 Capture ELISA IgM; rRT-PCR
(5)
OnSite Chikungunya IgM Combo Rapid Test CE NA 20 93 Capture ELISA IgM/IgG (in house); plaque reduction neutralization test
SD BIOLINE Chikungunya IgM test
 NA
 30
 73
 Capture ELISA IgM/IgG (in house); plaque reduction neutralization test
This study
OnSite Chikungunya IgM Combo Rapid Test CE 7 to 30 37.5 100 IFA IgM/IgG (commercial); rRT-PCR

*IFA, indirect immunofluorescence assay; NA, not applicable; rRT-PCR, real-time reverse transcription PCR.
†Manufacturers: CTK Biotech, San Diego, CA, USA (OnSite Chikungunya IgM Combo Rapid Test CE and OnSite Chikungunya IgM Rapid Test); Standard Diagnostics, Inc., Seoul, South Korea (SD BIOLINE Chikungunya IgM test).
‡Values are those reported in the original publications.
§Testing was done at 2 different time points after symptom onset.

Main Article

References
  1. Kosasih  H, Widjaja  S, Surya  E, Hadiwijaya  SH, Butarbutar  DP, Jaya  UA, Evaluation of two IgM rapid immunochromatographic tests during circulation of Asian lineage chikungunya virus. Southeast Asian J Trop Med Public Health. 2012;43:55–61.PubMedGoogle Scholar
  2. Rianthavorn  P, Wuttirattanakowit  N, Prianantathavorn  K, Limpaphayom  N, Theamboonlers  A, Poovorawan  Y. Evaluation of a rapid assay for detection of IgM antibodies to chikungunya. Southeast Asian J Trop Med Public Health. 2010;41:92–6.PubMedGoogle Scholar
  3. Yap  G, Pok  KY, Lai  YL, Hapuarachchi  HC, Chow  A, Leo  YS, Evaluation of chikungunya diagnostic assays: differences in sensitivity of serology assays in two independent outbreaks. PLoS Negl Trop Dis. 2010;4:e753. DOIPubMedGoogle Scholar
  4. Blacksell  SD, Tanganuchitcharnchai  A, Jarman  RG, Gibbons  RV, Paris  DH, Bailey  MS, Poor diagnostic accuracy of commercial antibody-based assays for the diagnosis of acute chikungunya infection. Clin Vaccine Immunol. 2011;18:1773–5. DOIPubMedGoogle Scholar
  5. Prat  CM, Flusin  O, Panella  A, Tenebray  B, Lanciotti  R, Leparc-Goffart  I. Evaluation of commercially available serologic diagnostic tests for chikungunya virus. Emerg Infect Dis. 2014;20:2129–32. DOIPubMedGoogle Scholar
  6. Okabayashi  T, Sasaki  T, Masrinoul  P, Chantawat  N, Yoksan  S, Nitatpattana  N, Detection of chikungunya virus antigen by a novel rapid immunochromatographic test. J Clin Microbiol. 2015;53:382–8. DOIPubMedGoogle Scholar

Main Article

Page created: September 19, 2016
Page updated: September 19, 2016
Page reviewed: September 19, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external