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Volume 22, Number 12—December 2016
Letter

Chlamydia-Related Bacteria in Free-Living and Captive Great Apes, Gabon

Anna Klöckner1, Michael Nagel1, Gilbert Greub, Sébastien Aeby, Karolin Hoffmann, Florian Liégeois, Francois Rouet, Stefania De Benedetti, Nicole Borel, and Beate HenrichfreiseComments to Author 
Author affiliations: University of Bonn, Bonn, Germany (A. Klöckner, S. De Benedetti, B. Henrichfreise); Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana (M. Nagel); University Hospital Center, Lausanne, Switzerland (G. Greub, S. Aeby); University of Lausanne, Lausanne (G. Greub, S. Aeby); University of Zurich, Zurich, Switzerland (K. Hoffmann, N. Borel); Institut de Recherche pour le Développement, Montpellier, France (F. Liégeois); Université de Montpellier 1, Montpellier (F. Liégeois); International Centre for Medical Research of Franceville, Franceville, Gabon (F. Liégeois, F. Rouet)

Main Article

Table

Analysis of 7 captive and free-living apes for Chlamydia-related bacteria by using real-time PCR and sequencing, Gabon*

Ape Source Species Mean Ct DNA copies/μL Closest BLAST† match for 16S rRNA gene Sequence identity, % Fragment size, bp E-value
Cola‡ Rectal swab Gorilla gorilla gorilla 33.02 10.57 Waddlia chondrophila WSU 86–1044, complete sequence 100 230 1 × 10–115
Cabinda‡ Penile swab Pan troglodytes troglodytes 33.32 8.58 W. chondrophila WSU 86–1044, complete sequence 98 241 1 × 10–111
Djela‡ Vaginal swab§ P. troglodytes troglodytes 29.34 122.41 Candidatus Rhabdochlamydia sp. cvE88, partial sequence 99 243 8 × 10–118
1882¶ Feces P. troglodytes troglodytes 34.29 8.24 Uncultured Chlamydiales CRG97, partial sequence 98 201 6 × 10–93
1883¶ Feces P. troglodytes troglodytes 31.90 43.55 Uncultured Chlamydiales CRG97, partial sequence 99 200 1 × 10–95
1885¶ Feces P. troglodytes troglodytes 31.16 73.41 Uncultured Chlamydiales CRG97, partial sequence 96 209 1 × 10–90
Gab2130¶ Feces G. gorilla gorilla 35.30 2.38 W. chondrophila WSU 86–1044, complete sequence 100 218 5 × 10–109

*Ct, cycle threshold.
†http://blast.ncbi.nlm.nih.gov/Blast.cgi
‡Captive ape.
§A second vaginal swab specimen from the same chimpanzee that was collected 1 mo later still showed a positive result by real-time PCR, and sequencing indicated the presence of a Candidatus Rhabdochlamydia sp. cvE88.
¶Free-living ape.

Main Article

1These authors contributed equally to this article.

Page created: November 18, 2016
Page updated: November 18, 2016
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