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Volume 22, Number 12—December 2016
Letter

Possible Foodborne Transmission of Hepatitis E Virus from Domestic Pigs and Wild Boars from Corsica

Nicole PavioComments to Author , Morgane Laval, Oscar Maestrini, François Casabianca, François Charrier, and Ferran Jori
Author affiliations: ANSES (French Agency for Food, Environmental and Occupational Health and Safety); Maisons-Alfort, France (N. Pavio); INRA (National Institute for Agricultural Research); Maisons-Alfort (N. Pavio); University Paris 12, National Veterinary School, Maisons-Alfort (N. Pavio); INRA, Corte, France (M. Laval, O. Maestrini, F. Casabianca, F. Charrier); CIRAD (Agricultural Research for Development); Montpellier, France (F. Jori); Botswana University of Agriculture and Natural Resources, Gaborone, Botswana (F. Jori)

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Figure

Phylogenetic tree of hepatitis E virus (HEV) sequences identified in samples from wild boars and pigs from Corsica. All 10 HEV sequences (GenBank accession nos. KT334188–KT334197) corresponding to the open reading frame 2 capsid nucleotides 6044–6334 of the reference sequence AF082843, were obtained by Sanger dideoxy sequencing, from wild boars (WB, black circles) or pigs (S, triangles). Sequences were aligned with Muscle (MEGA6, http://www.megasoftware.net) with the 5 closest sequences (retriev

Figure. Phylogenetic tree of hepatitis E virus (HEV) sequences identified in samples from wild boars and pigs from Corsica. All 10 HEV sequences (GenBank accession nos. KT334188–KT334197) corresponding to the open reading frame 2 capsid nucleotides 6044–6334 of the reference sequence AF082843, were obtained by Sanger dideoxy sequencing, from wild boars (WB, black circles) or pigs (S, triangles). Sequences were aligned with Muscle (MEGA6, http://www.megasoftware.net) with the 5 closest sequences (retrieved by using BLAST, http://blast.ncbi.nlm.nih.gov/Blast.cgi) and reference sequences (9). The closest HEV sequences from France (gray circles; Hu: human, FIG: figatellu) are identified with their GenBank accession number and year of detection. The tree was constructed by using the neighbor-joining method with a bootstrap of 1,000 replicates. Bootstrap values >70% are indicated on respective branches. Three distinct clusters (1–3) are indicated at right. GenBank reference sequences suggested by Smith et al. for genotype subtyping (9): 3a, AF082843; 3b, AP003430; 3c, FJ705359; 3e, AB248521; 3f, AB369687; 3g, AF455784; 3h, JQ013794; 3i, FJ998008; 3j, AY115488; AB290312, JQ953664, AB369689, AB290313, EU360977, KJ873911, EU723513. Countries of origin of the sequences used are as follows: CA, Canada; FR, France; GER, Germany; InJAVA, Indonesia; IT, Italy; JP, Japan; KY, Kyrgyzstan; MG, Mongolia; NL, the Netherlands; NW, Norway; SP, Spain; SW, Sweden; TH, Thailand; UK, United Kingdom; USA, United States. Scale bar represents nucleotide substitutions per site.

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