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Volume 22, Number 4—April 2016
Research

Evaluation of Viremia Frequencies of a Novel Human Pegivirus by Using Bioinformatic Screening and PCR

David Bonsall, William F. Gregory, Camilla L.C. Ip, Sharyne Donfield, James Iles, M. Azim Ansari, Paolo Piazza, Amy Trebes, Anthony Brown, John Frater, Oliver G. Pybus, Phillip Goulder, Paul Klenerman, Rory Bowden, Edward D. Gomperts, Eleanor Barnes, Amit Kapoor, Colin P. Sharp, and Peter SimmondsComments to Author 
Author affiliations: University of Oxford, Oxford, UK (D. Bonsall, C.L.C. Ip, J. Iles, M.A. Ansari, P. Piazza, A. Trebes, A. Brown, J. Frater, O.G. Pybus, P. Goulder, P. Klenerman, R. Bowden, E. Barnes, P. Simmonds); University of Edinburgh Easter Bush, Edinburgh, Scotland, UK (W.F. Gregory, C.P. Sharp); Rho, Inc., Chapel Hill, North Carolina, USA (S. Donfield); The Childrens' Hospital, Los Angeles, California, USA (E.D. Gomperts); The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA (A. Kapoor)

Main Article

Table 4

Validation of human pegivirus detection in plasma samples by using PCR*

No. samples
Metagenomic screen results†
Primers, no. positive
HHpgV-1
HPgV
Group 2
ak1/ak2‡
Group 1
2 + 2 2 0
1 +/– 0 0 0
10 + 0 0 10
10



0
0
0
*HPgV, human pegivirus; HHpgV, human hepegivirus; +, positive; ­–, negative.
†Virus status as determined by bioinformatic screening.
‡Nonstructural 3 genes as previously described (6).

Main Article

Page created: March 15, 2016
Page updated: March 15, 2016
Page reviewed: March 15, 2016
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