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Volume 22, Number 5—May 2016
Research

Plasmodium falciparum K76T pfcrt Gene Mutations and Parasite Population Structure, Haiti, 2006–2009

Macarthur Charles, Sanchita Das, Rachel Daniels, Laura Kirkman, Glavdia G. Delva, Rodney Destine, Ananias A. Escalante, Leopoldo Villegas, Noah M. Daniels, Kristi Shigyo, Sarah K. Volkman, Jean W. Pape, and Linnie M. GolightlyComments to Author 
Author affiliations: Weill Medical College of Cornell University, New York, New York, USA (M. Charles, S. Das, L. Kirkman, K. Shigyo, J.W. Pape, L.M. Golightly); The Haitian Group for the Study of Kaposi's Sarcoma and Opportunistic Infections Centers (GHESKIO); Port-au-Prince, Haiti (M. Charles, G.G. Delva, R. Destine, J.W. Pape); Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA (R. Daniels, S.K. Volkman); Broad Institute, Cambridge, Massachusetts, USA (R. Daniels, S.K. Volkman); Temple University, Department of Biology, Philadelphia, Pennsylvania, USA (A. Escalante); ICF International, Rockville, Maryland, USA (L. Villegas); Centro de Investigación de Campo Dr. Francesco Vitanza, Tumeremo, Venezuela (L. Villegas); Massachusetts Institute of Technology, Cambridge (N.M. Daniels); Simmons College, Boston (S.K. Volkman)

Main Article

Table 1

Evaluation of Plasmodium falciparum samples for chloroquine resistance by restriction-endonuclease digestion and sequencing, Haiti, 2006–2009*

Source No. samples No. pfcrt K76T Apo1 sensitive No. pfcrt K76T Apo1 combined No. pfcrt K76T sequenced CVMNK No. pfcrt K76T sequenced CVMNK/CVIET
Jeremie 308 73 1† 234 1†
Jacmel 36 14 0 22 0
Les Cayes 316 40 1‡ 275 1‡
Miragoane 15 7 0 8 0
Cap-Haitien 4 2 0 2 0
Deschappelles 199 20 0 179 0
Fort-Liberte 13 ND NA 13 0
Port-de-Paix 3 ND NA 3 0
Port-au-Prince
 7
 ND
 NA
 7
 0
Total 901 156 2 743 2

*Sequenced includes sequencing of the nested PCR product for pfcrt haplotypes where wild-type haplotype = CVMNK, and mutant haplotype = CVIET;
ND, not determined; NA, not applicable; pfcrt, P. falciparum chloroquine resistance transporter.
†Same sample from Jeremie.
‡Same sample from Les Cayes; pfcrt K76T ApoI includes endonuclease digestion and gel electrophoresis. Sensitive: K76; resistant: 76T; pfcrt K76T.

Main Article

Page created: April 13, 2016
Page updated: April 13, 2016
Page reviewed: April 13, 2016
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