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Volume 22, Number 8—August 2016
Dispatch

Human Tick-Borne Encephalitis and Characterization of Virus from Biting Tick

Anna J. HenningssonComments to Author , Richard Lindqvist, Peter Norberg, Pontus Lindblom, Anette Roth, Pia Forsberg, Tomas Bergström, Anna K. Överby, and Per-Eric Lindgren
Author affiliations: Region Jönköping County, Jönköping, Sweden (A.J. Henningsson, P.-E. Lindgren); Umeå University, Umeå, Sweden (R. Lindqvist, A.K. Överby); University of Gothenburg, Gothenburg, Sweden (P. Norberg, A. Roth, T. Bergström); Linköping University, Linköping, Sweden (P. Lindblom, P. Forsberg, P.-E. Lindgren); Linköping University Hospital, Linköping (P. Forsberg)

Main Article

Figure 2

Time course of tick-borne encephalitis virus (TBEV) multiplication from sample from a 67-year-old man in Sweden, 2011. A549 cells were infected with the virus isolated from the tick in this study, tick/SWE/Habo/2011/1, and reference strain Hypr at multiplicity of infection 0.1. Total cellular RNA and cell culture supernatants were collected at different time points postinfection. A) Intracellular levels of viral RNA quantified by real-time reverse transcription PCR analysis. B) Virus titers in c

Figure 2. Time course of tick-borne encephalitis virus (TBEV) multiplication from sample from a 67-year-old man in Sweden, 2011. A549 cells were infected with the virus isolated from the tick in this study, tick/SWE/Habo/2011/1, and reference strain Hypr at multiplicity of infection 0.1. Total cellular RNA and cell culture supernatants were collected at different time points postinfection. A) Intracellular levels of viral RNA quantified by real-time reverse transcription PCR analysis. B) Virus titers in cell culture supernatants as determined by focus-forming assay (FFU). Mean values from 3 independent experiments are shown; error bars indicate SD. p values determined by Student t test.

Main Article

Page created: July 15, 2016
Page updated: July 15, 2016
Page reviewed: July 15, 2016
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