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Volume 4, Number 1—March 1998

Dispatch

Laboratory Survey of Drug-Resistant Streptococcus pneumoniae in New York City, 1993—1995

Richard Heffernan, Kelly Henning, Anne Labowitz, Annette Hjelte, and Marcelle Layton
Author affiliations: New York City Department of Health, New York, New York, USA

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Table 2

Penicillin susceptibility testing protocols for Strepococcus pneumoniae in New York City hospital laboratories

No. of laboratoriesa 1993
No. (%) 1994
No. (%) 1995
No. (%)
Screened with oxacillin disk 55 (82) 63 (97) 65 (97)
Screened all isolates 38 (57) 56 (86) 59 (88)
Screened only sterile 9 (13) 6 (9) 6 (9)
Other 8 (12) 1 (2) 0
Performed penicillin
MIC test
MIC-tested 39 (58) 51 (78) 56 (84)
All isolates 10 (15) 9 (14) 7 (10)
Oxacillin-resistant only 18 (27) 39 (60) 42 (63)
Sterile isolates only 5 (7) 1 (2) 0 (0)
By request only 4 (6) 1 (2) 5 (7)
Other 2 (3) 1 (2) 2 (3)
Penicillin MIC techniqueb Antibiotic gradient stripc 11(16) 41 (63) 50 (75)
Automated testsc 21 (31) 9 (14) 2 (3)
Broth dilution 8 (12) 11 (17) 5 (7)
Unknown 2 (3) 3 (5) 0
Conformed with NCCLS guidelinesd 15 (22) 38 (58) 46 (69)

aTotal number of laboratories responding to survey in 1993, 1994, 1995 was 67, 65, 67, respectively.
b13 laboratories used more than one MIC technique.
cAntibiotic gradient strip: e.g., E-test. Automated tests: e.g., Microscan, Vitek.
dNCCLS=National Committee for Clinical Laboratory Standards. Screened all S. pneumoniae isolates with oxacillin disk diffusion test and confirmed resistance with approved penicillin MIC test.

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