Volume 5, Number 1—February 1999
Research
Genetic Diversity and Distribution of Peromyscus-Borne Hantaviruses in North America
Martha C. Monroe*, Sergey P. Morzunov†, Angela M. Johnson*, Michael D. Bowen*, Harvey Artsob‡, Terry Yates§, C.J. Peters*, Pierre E. Rollin*, Thomas G. Ksiazek*, and Stuart T. Nichol*
Table 2
PCR and sequencing primers
| 1st-round primers (5' to 3') | 2nd-round primers (5' to 3') | Basis of primer design (ref.) | Amplicon size |
|---|---|---|---|
| TTTAAGCAATGGTG(C/T)ACTAC(T/A)AC | AGAAAGAAATGTGCATTTGC | Puumala/ Prospect Hill/ | 278 |
| CCATAACACAT(A/T)GCAGC | CCTGAACCCCATGC(A/T/C)CCATC | Arvicolinae | (2) |
| TTTAAGCAATGGTG(C/T)ACTAC(T/A)AC | AAGGTAACACAGT(G/C)TCTGGATTC | Sin Nombre/ Western | 185 |
| CCATAACACAT(A/T)GCAGC | GGTTATCACTTAGATC(C/T)TGAAAGG | U.S. 1st generation | (2) |
| AGAAAGATCTGTGGGTTTGC | AAGGTAACACAGT(G/C)TCTGGATTC | Sin Nombre/ Western | 185 |
| CCTGAACCCCAGGCCCCGT | GGTTATCACTTAGATC(C/T)TGAAAGG | U.S. 2nd generation | (11) |
| TGTGTGTTTGGAGACCCTGG | ATGTCAACAAC(A/G)AGTGGGATG | Sin Nombre Nevada/ | 185 |
| TC(A/G)ATAGATTGTGTATGCA | CATGGGTTATCACTTAG(G/A)TC | E. California | (31) |
| CAGAAAGATCTGCGGGTTTGC | CAAGGGAATACTGTCTCTGGATTT | Bayou virus/LA/ | 185 |
| CCCGAGCCCCATGCACCAT | GATTGTCACTCAGATCTTGAAATG | East Coast/ S. American (19,20) | |
| TGTGAITATCAAGGIAAIAC | TGTGAITATCAAGGIAAIAC | General Sigmodontinae | 242 |
| ACIG(A/T)IGCICCATAICACAT | CCCCAIGCICCITCAAT | (10) |
