Volume 6, Number 1—February 2000
Research
Norwalk-Like Calicivirus Genes in Farm Animals
Wim H.M. van der Poel*
, Jan Vinjé*, Reina van der Heide*, Maria-Inmaculada Herrera†, Amparo Vivo†, and Marion P.G. Koopmans*
, Jan Vinjé*, Reina van der Heide*, Maria-Inmaculada Herrera†, Amparo Vivo†, and Marion P.G. Koopmans*Table
Viruses and virus particles detected by electron microscopy and corresponding Norwalk-like virus (NLV) RT-PCR results (including hybridization with a calf-NLV specific probe) in 16 of the 75 calf herd samples
| Sample | RT-PCRa results | EM results |
|---|---|---|
| CH121b | Neg | Adenovirus |
| CH127 | Neg | Parvovirus-like (21 nm) Flavivirus-like particles(34-44 nm) |
| CH128 | Neg | Parvovirus-like (19 nm) Rotavirus |
| CH141 | Neg | BDV-likec enveloped spherical particles (47 nm) |
| CH145 | Neg | Parvovirus-like (21 nm) |
| CH153 | Pos | Rotavirus |
| CH155 | Neg | Rotavirus |
| CH156 | Pos | Parvovirus-like (25-27 nm) |
| CH161 | Pos | Rotavirus |
| CH168 | Pos | Coronavirus-like particles |
| CH172 | Neg | Parvovirus-like (21 nm) |
| CH176 | Pos | SRSVd |
| CH178 | Pos | Rotavirus |
| CH185 | Pos | Rotavirus |
| CH186 | Neg | Rotavirus |
| CH187 | Neg | Coronavirus-like particles |
aRT-PCR = reverse transcription-polymerase chain reaction;
bCH = calf herd; cBDV-like: bovine diarrhea viruslike.;
dSRSV: small round-structured virus or NLV.
