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Volume 6, Number 1—February 2000

Research

Norwalk-Like Calicivirus Genes in Farm Animals

Wim H.M. van der Poel*Comments to Author , Jan Vinjé*, Reina van der Heide*, Maria-Inmaculada Herrera†, Amparo Vivo†, and Marion P.G. Koopmans*
Author affiliations: *National Institute of Public Health and the Environment, Bilthoven, the Netherlands; †Instituto de Salud Carlos III, Majadahonda, Madrid, Spain

Main Article

Table

Viruses and virus particles detected by electron microscopy and corresponding Norwalk-like virus (NLV) RT-PCR results (including hybridization with a calf-NLV specific probe) in 16 of the 75 calf herd samples

Sample RT-PCRa results EM results
CH121b Neg Adenovirus
CH127 Neg Parvovirus-like (21 nm) Flavivirus-like particles(34-44 nm)
CH128 Neg Parvovirus-like (19 nm) Rotavirus
CH141 Neg BDV-likec enveloped spherical particles (47 nm)
CH145 Neg Parvovirus-like (21 nm)
CH153 Pos Rotavirus
CH155 Neg Rotavirus
CH156 Pos Parvovirus-like (25-27 nm)
CH161 Pos Rotavirus
CH168 Pos Coronavirus-like particles
CH172 Neg Parvovirus-like (21 nm)
CH176 Pos SRSVd
CH178 Pos Rotavirus
CH185 Pos Rotavirus
CH186 Neg Rotavirus
CH187 Neg Coronavirus-like particles

aRT-PCR = reverse transcription-polymerase chain reaction;
bCH = calf herd; cBDV-like: bovine diarrhea viruslike.;
dSRSV: small round-structured virus or NLV.

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