Volume 6, Number 6—December 2000
Hantavirus Pulmonary Syndrome Associated with Monongahela Virus, Pennsylvania
1Three fragments of the virus genome were amplified by nested polymerase chain reaction to allow generation of nucleotide (nt) sequences 393 nt in length for an S segment region encoding the N protein, 259 nt in length for an M segment region (M-G1) encoding the G1 protein, and 205 nt in length for an M segment region (M-G2) encoding the G2 protein (12). Sequence data were analyzed by using the Wisconsin GCG Package (version 9.1) software. Initial multiple sequence alignment was done with the GCG Pileup program, and phylogenetic analysis was performed by using the Phylogenetic Analysis Using Parsimony (and other methods) program (version 4.0) (20).