Waterborne Outbreak of Tularemia Associated with Crayfish Fishing
Pedro Anda* , Javier Segura del Pozo*†, José María Díaz García‡, Raquel Escudero*, F. Javier García Peña§, M. Carmen López Velasco‡, Ricela E. Sellek*, M. Rosario Jiménez Chillarón‡, Luisa P. Sánchez Serrano*, and J. Fernando Martínez Navarro*
Author affiliations: *Instituto de Salud Carlos III, Majadahonda, Madrid, Spain; †Public Health Department, Alcalá de Henares, Madrid, Spain; ‡Cuenca Public Health Department, Cuenca, Spain; §Ministry of Agriculture and Fisheries, Algete, Madrid, Spain
Figure 4. . Polymerase chain reaction results in selected samples from water, crayfish, patients, and controls. Lanes 1 and 2: positive control (first [1550-bp] and second [950-bp] rounds of amplification); lanes 3 and 4: water samples 1 and 3, respectively, from the sewage plant, second round; lanes 5 and 6: stomach and hepatopancreas, respectively, from batch A of crayfish, second round; lane 7: negative control; lanes 8 and 10: control of amplification of 1 CFU for the first and second rounds of amplification, respectively; lanes 11 and 12: human lymph node aspirate, first and second amplifications, respectively; lanes 13 and 14: positive control, first round; lanes 15-19,21, and 22: water samples from the river; lanes 23 and 24: stomach and hepatopancreas, respectively, from batch B of crayfish; lanes 25 and 26: pooled DNA from Salmonella Typhimurium, Escherichia coli, Legionella pneumophila, Yersinia enterocolitica, and Proteus vulgaris (first and second rounds of amplification, respectively); lanes 9 and 20, 1-kb DNA ladder size standards.
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