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Volume 8, Number 10—October 2002
THEME ISSUE
Bioterrorism-related Anthrax

Bioterrorism-related Anthrax

Molecular Subtyping of Bacillus anthracis and the 2001 Bioterrorism-Associated Anthrax Outbreak, United States

Alex R. Hoffmaster*Comments to Author , Collette C. Fitzgerald*, Efrain Ribot*, Leonard W. Mayer*, and Tanja Popovic*
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA;

Main Article

Table 1

Primers used for amplification and sequencing of the pagA gene

Primer Sequence (5´ – 3´)
PA1575Fa CGA ACT GAT ACA CGT ATT TTA G
PA4191Ra AGG ATT ATG ATG ATT TAG ATT ACT
PA1566Fa TTT ATC CGA ACT GAT ACA CGT ATT
PA4205Ra ACA AAC AAT CTC AAA GGA TTA TGA
PA-1Fa ATA TTT ATA AAA GTT CTG TTT AAA AAG CC
PA-1Ra TAA ATC CTG CAG ATA CAC TCC CAC
PA-2Fa ATA AGT AAA AAT ACT TCT ACA AGT AGG ACA C
PA-2Ra GAT TTA GAT TAC TGT TTA AAA CAT ACT CTC C
PA-3 TCA TGT AAC AAT GTG GGT AGA TGA C
PA-4 CTC TAT GAG CCT CCT TAA CTA CTG AC
PA-5F ATC CTA GTG ATC CAT TAG AAA CGA C
PA-5R CTT CTC TAT GAG CCT CCT TAA CTA CTG
PA-5Fnest AGT GAT CCA TTA GAA ACG AC
PA-5Rnest TAA CTA CTG ACT CAT CCG C
PA-U2121 TAC ATT TGC TAC TTC CGC TGA TAA
PA-L3892 TGT TTT TCC ATC TTG CCG TAA
2121R TTA TCA GCG GAA GTA GCA AAT GTA
3892F TTA CGG CAA GAT GGA AAA ACA
2557R AGC CGT GCT CCA TTT TTC AGG
3318R TGC GGT AAC ACT BTCA CTC CAG
2560F GAA AAA TGG AGC ACG GCT TCT
2924F CTT GGG CTG AAA CAA TGG GTT

aPrimers used for amplification of the pagA gene. All other primers were used for sequencing.

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