Volume 8, Number 10—October 2002
THEME ISSUE
Bioterrorism-related Anthrax

Bioterrorism-related Anthrax

Molecular Subtyping of Bacillus anthracis and the 2001 Bioterrorism-Associated Anthrax Outbreak, United States

Alex R. Hoffmaster* , Collette C. Fitzgerald*, Efrain Ribot*, Leonard W. Mayer*, and Tanja Popovic*

Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA;

Main Article

Table 1

Primers used for amplification and sequencing of the pagA gene

Primer	Sequence (5´ – 3´)
PA1575Fa	CGA ACT GAT ACA CGT ATT TTA G
PA4191Ra	AGG ATT ATG ATG ATT TAG ATT ACT
PA1566Fa	TTT ATC CGA ACT GAT ACA CGT ATT
PA4205Ra	ACA AAC AAT CTC AAA GGA TTA TGA
PA-1Fa	ATA TTT ATA AAA GTT CTG TTT AAA AAG CC
PA-1Ra	TAA ATC CTG CAG ATA CAC TCC CAC
PA-2Fa	ATA AGT AAA AAT ACT TCT ACA AGT AGG ACA C
PA-2Ra	GAT TTA GAT TAC TGT TTA AAA CAT ACT CTC C
PA-3	TCA TGT AAC AAT GTG GGT AGA TGA C
PA-4	CTC TAT GAG CCT CCT TAA CTA CTG AC
PA-5F	ATC CTA GTG ATC CAT TAG AAA CGA C
PA-5R	CTT CTC TAT GAG CCT CCT TAA CTA CTG
PA-5Fnest	AGT GAT CCA TTA GAA ACG AC
PA-5Rnest	TAA CTA CTG ACT CAT CCG C
PA-U2121	TAC ATT TGC TAC TTC CGC TGA TAA
PA-L3892	TGT TTT TCC ATC TTG CCG TAA
2121R	TTA TCA GCG GAA GTA GCA AAT GTA
3892F	TTA CGG CAA GAT GGA AAA ACA
2557R	AGC CGT GCT CCA TTT TTC AGG
3318R	TGC GGT AAC ACT BTCA CTC CAG
2560F	GAA AAA TGG AGC ACG GCT TCT
2924F	CTT GGG CTG AAA CAA TGG GTT

^aPrimers used for amplification of the pagA gene. All other primers were used for sequencing.

Main Article

Top of Page