Emerging Infectious Disease ISSN: 1080-6059

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Molecular Subtyping of Bacillus anthracis and the 2001 Bioterrorism-Associated Anthrax Outbreak, United States

Alex R. Hoffmaster*

, Collette C. Fitzgerald*, Efrain Ribot*, Leonard W. Mayer*, and Tanja Popovic*

Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA;

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Table 2

MLVA and pagA genotyping of Bacillus anthracis isolates^a,b

B. anthracis strain	No. strains	vrrA	vrrB₁	vrrB₂	vrrC₁	vrrC₂	CG3	pXO1	pXO2	MLVA type	PA genotype
Outbreak-associated	317	313	229	153	583	532	158	123	141	62	I
Ames	1	313	229	153	583	532	158	123	141	62	I
NH (2000032764)	1	301	229	153	538	604	158	132	139	78	VI
NH (2000032760)	1	313	229	153	538	604	158	123	139	New^e	VI
RI (2000032763)	1	313	229	162	538	604	158	132	139	71	VI
RI (2000032761)	1	313	229	162	538	604	158	132	139	71	VI
MA (2000032762)	1	313	229	153	538	604	158	132	143	New^e	VI
State A (2002017388)	1	313	229	162	613	604	153	–	137	Pasteur^c	NA^d
Pasteur (LRN)	1	313	229	162	613	604	153	–	137	Pasteur^c	NA^d
Country B (2002007581)	1	313	229	162	613	532	158	129	141	45	I
Country B (2002007648)	1	313	229	162	613	532	158	129	141	45	I
Country B (2002007649)	1	313	229	162	613	604	153	–	137	Pasteur^c	NA^d
Country B (2002007650)	1	313	229	162	583	532	158	129	_	Sterne^c	I
Country B (2002007651)	1	313	229	162	583	532	158	129	_	Sterne^c	I

^aMLVA, multiple-locus variable-number tandem repeat analysis; pagA, protective antigen gene; PA, protective antigen; –, loci not detected; NA, not applicable.
^bAllele size for each VNTR locus is shown in addition to the MLVA and PA genotypes.
^cNo MLVA genotype assigned due to the lack of one of the virulence plasmids (pXO1 or pXO2).
^dpagA not present in pXO1-cured strains and thus could not be assigned a PA genotype.
^eNew combination of alleles resulting in a new genotype. Genotype no. to be assigned at a later date.

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