Volume 8, Number 12—December 2002
Research
First Isolation of West Nile virus from a Patient with Encephalitis in the United States
Cinnia Huang*
, Brett Slater*, Robert Rudd*, Nandakishore Parchuri†, Rene Hull*, Michelle Dupuis*, and Alexander Hindenburg†
, Brett Slater*, Robert Rudd*, Nandakishore Parchuri†, Rene Hull*, Michelle Dupuis*, and Alexander Hindenburg†Table 2
Detection of West Nile virus in human specimens by TaqMan and standard RT-PCR assaysa
| Specimen | Collection date | RNA (copy/mL) | CT | Rn | STD RT-PCR | Serology | Cell cultures |
|---|---|---|---|---|---|---|---|
| Blood | 9/21/2001 | 2.2 x 103 | 31.6 | 0.48 | Positive | n.d. | n.d. |
| Blood | 9/24/2001 | 2.5 x 106 | 20.4 | 3.71 | Positive | n.d. | Positive |
| CSF | 9/26/2001 | 1.1 x 106 | 20.4 | 3.71 | Positive | Negative | Negative |
| Blood | 10/02/2001 | 5.4 x 104 | 25.8 | 2.70 | Positive | n.d. | n.d. |
| Serum | 10/10/2001 | 3.7 x 103 | 28.5 | 0.70 | Positive | Negative | n.d. |
aCT, threshold cycle number, the cycle number at which fluorescence increases above a fixed threshold value; Rn, normalized fluorescent signal, the fluorescent signal generated by the reporter dye; STD, standard; RT-PCR, reverse transcription–polymerase chain reaction; n.d.: not done.
