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Volume 8, Number 12—December 2002

Research

First Isolation of West Nile virus from a Patient with Encephalitis in the United States

Cinnia Huang*Comments to Author , Brett Slater*, Robert Rudd*, Nandakishore Parchuri†, Rene Hull*, Michelle Dupuis*, and Alexander Hindenburg†
Author affiliations: *Wadsworth Center, New York State Department of Health, Albany, New York, USA; †Winthrop University Hospital, Mineola, New York, USA;

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Table 2

Detection of West Nile virus in human specimens by TaqMan and standard RT-PCR assaysa

Specimen Collection date RNA (copy/mL) CT Rn STD RT-PCR Serology Cell cultures
Blood 9/21/2001 2.2 x 103 31.6 0.48 Positive n.d. n.d.
Blood 9/24/2001 2.5 x 106 20.4 3.71 Positive n.d. Positive
CSF 9/26/2001 1.1 x 106 20.4 3.71 Positive Negative Negative
Blood 10/02/2001 5.4 x 104 25.8 2.70 Positive n.d. n.d.
Serum 10/10/2001 3.7 x 103 28.5 0.70 Positive Negative n.d.

aCT, threshold cycle number, the cycle number at which fluorescence increases above a fixed threshold value; Rn, normalized fluorescent signal, the fluorescent signal generated by the reporter dye; STD, standard; RT-PCR, reverse transcription–polymerase chain reaction; n.d.: not done.

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