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Volume 8, Number 3—March 2002

Research

Novel Cryptosporidium Genotypes in Sporadic Cryptosporidiosis Cases: First Report of Human Infections with a Cervine Genotype

Corinne S. L. Ong*†Comments to Author , Diane L. Eisler*, Alireza Alikhani*, Vicki W. K. Fung*, Joan Tomblin‡, William R. Bowie*, and Judith L. Isaac-Renton*†
Author affiliations: *University of British Columbia, Vancouver, British Columbia; †BC Centre for Disease Control, Vancouver, British Columbia; ‡BC Biomedical Laboratories Ltd., Surrey, British Columbia, Canada.;

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Figure 1

Restriction profiles obtained after digestion of polymerase chain reaction products from the ITS1 locus with Mse I. lanes 1- 3-, 100-, 50-, and 10-bp ladder molecular weight markers; lanes 4 and 15, bovine genotype 2 isolates; lanes 5, 6, 14, and 16, human genotype 1 isolates including DE340 (lane 14); lanes 7 and 9–12, cervine genotype isolates including MH205 (lane 7), TK320 (lane 10), and DE302 (lane 11); lane 8, Cryptosporidium meleagridis isolate CS33; lanes 13 and 17, other novel genotype isolates such as VF383 (lane 13) and TK348 (lane 17)

Figure 1. Restriction profiles obtained after digestion of polymerase chain reaction products from the ITS1 locus with Mse I. lanes 1- 3-, 100-, 50-, and 10-bp ladder molecular weight markers; lanes 4 and 15, bovine genotype 2 isolates; lanes 5, 6, 14, and 16, human genotype 1 isolates including DE340 (lane 14); lanes 7 and 9–12, cervine genotype isolates including MH205 (lane 7), TK320 (lane 10), and DE302 (lane 11); lane 8, Cryptosporidium meleagridis isolate CS33; lanes 13 and 17, other novel genotype isolates such as VF383 (lane 13) and TK348 (lane 17)

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