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Volume 8, Number 4—April 2002

Research

Genetic and Antigenic Analysis of the First A/New Caledonia/20/99-like H1N1 Influenza Isolates Reported in the Americas

Luke T. Daum*, Linda C. Canas*, Catherine B. Smith†, Alexander Klimov†, William Huff*, William Barnes*, and Kenton L. Lohman*Comments to Author 
Author affiliations: *Brooks Air Force Base, San Antonio, Texas, USA; †Centers for Disease Control and Prevention, Atlanta, Georgia, USA;

Main Article

Figure 2

An unrooted phylogenetic analysis of the HA1 gene nucleotide sequence of influenza A H1N1 viruses isolated since 1990. Peru isolates 1621 and 1798 are within the Beijing/262/95 lineage but are more similar to A/New Caledonia/20/99. A number of isolates characterized by Brooks Air Force Base from 1999 to 2001 were also A/NC/20/99-like. The tree was generated by using the Jotun-Hein algorithm (6) in MegAlign software (version 3.18). Horizontal lines are proportional to the number of substitutions between branch points. Asterisk (*) denotes 2000/01 vaccine strain; (1) denotes isolates characterized by Brooks Air Force Base. Brooks Air Force Base isolates are available from Genbank under accession numbers AF268312, AF268313 and AY029287-AY029292.

Figure 2. An unrooted phylogenetic analysis of the HA1 gene nucleotide sequence of influenza A H1N1 viruses isolated since 1990. Peru isolates 1621 and 1798 are within the Beijing/262/95 lineage but are more similar to A/New Caledonia/20/99. A number of isolates characterized by Brooks Air Force Base from 1999 to 2001 were also A/NC/20/99-like. The tree was generated by using the Jotun-Hein algorithm (6) in MegAlign software (version 3.18). Horizontal lines are proportional to the number of substitutions between branch points. Asterisk (*) denotes 2000/01 vaccine strain; (1) denotes isolates characterized by Brooks Air Force Base. Brooks Air Force Base isolates are available from Genbank under accession numbers AF268312, AF268313 and AY029287-AY029292.

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