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Volume 8, Number 7—July 2002

Research

First Human Isolate of Hantavirus (Andes virus) in the Americas

Hector Galeno*, Judith Mora*, Eliecer Villagra*, Jorge Fernandez*, Jury Hernandez†, Gregory J. Mertz‡, and Eugenio Ramirez*Comments to Author 
Author affiliations: *Public Health Institute of Chile, Santiago, Chile; †Los Angeles Hospital, Santiago, Chile; ‡University of New Mexico Health Sciences Center, Albuquerque, New Mexico, USA;

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Figure 2

Maximum-parsimony tree analysis comparing S nucleotide sequence of CHI-7913 virus with homologous sequences of previously characterized hantaviruses. Nucleotide sequences examined correspond to positions 22–359 of antigenome-sense sequence of nucleoprotein (N) gene. Sequences were analyzed by the maximun parsimony method with the Clustal W and PHYLIP packages (15). The minimal length trees shown were supported as the majority rule consensus tree in 500 replicates. The bootstrap replicates supporting each node are indicated. References and GenBank accession numbers for the sequences used in S segment comparisons are BCC (16) L39949; BAY (17) L36929; NY strain RI-1 (18), U09488; SN strain cc107 (19), L33683; SN strain nmh10 (20), L25784; PH strain PH-1 (21), and M34011; Puumala strains Sotkamo (22), X61035; Seoul (SEO) strain sr-11 (23), and M34881; Hantaan (HTN) strain 76-118 (24), M14626; Andes strain AH-1 (14), AF004660; ESQ H-1/96 (14), AF005948; CH H-1/96 (14), AF 005947; AND Nort (strain unpublished) AF325966; and Andes strain 23 (AF291702).

Figure 2. Maximum-parsimony tree analysis comparing S nucleotide sequence of CHI-7913 virus with homologous sequences of previously characterized hantaviruses. Nucleotide sequences examined correspond to positions 22–359 of antigenome-sense sequence of nucleoprotein (N) gene. Sequences were analyzed by the maximun parsimony method with the Clustal W and PHYLIP packages (15). The minimal length trees shown were supported as the majority rule consensus tree in 500 replicates. The bootstrap replicates supporting each node are indicated. References and GenBank accession numbers for the sequences used in S segment comparisons are BCC (16) L39949; BAY (17) L36929; NY strain RI-1 (18), U09488; SN strain cc107 (19), L33683; SN strain nmh10 (20), L25784; PH strain PH-1 (21), and M34011; Puumala strains Sotkamo (22), X61035; Seoul (SEO) strain sr-11 (23), and M34881; Hantaan (HTN) strain 76-118 (24), M14626; Andes strain AH-1 (14), AF004660; ESQ H-1/96 (14), AF005948; CH H-1/96 (14), AF 005947; AND Nort (strain unpublished) AF325966; and Andes strain 23 (AF291702).

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