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Volume 8, Number 8—August 2002

Research

DNA Vaccine Expressing Conserved Influenza Virus Proteins Protective Against H5N1 Challenge Infection in Mice

Suzanne L. Epstein*Comments to Author , Terrence M. Tumpey†, Julia A. Misplon*, Chia-Yun Lo*, Lynn A. Cooper‡, Kanta Subbarao‡, Mary Renshaw‡, Suryaprakash Sambhara‡, and Jacqueline M. Katz‡
Author affiliations: *Food and Drug Administration Rockville, Maryland, USA; †United States Department of Agriculture, Athens, Georgia, USA; ‡Centers for Disease Control and Prevention, Atlanta, Georgia, USA;

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Table

Effect of DNA vaccination on replication of HK/486 challenge virus in mouse lungsa

Immunization No. mice Lung titer +/- SE
Expt 1A
A/NP+A/M DNA 6 5.7 ± 0.33
B/NP + blank DNA 6 6.9 ± 0.18b
None 6 6.9 ± 0.22c
Expt 1B
Live X-31 virus 4 3.6 ± 0.36d
None 4 7.1 ± 0.1

aMice were immunized intramuscularly with 100 μg each of influenza A nucleoprotein and matrix DNA (A/NP+A/M DNA) or controls with 100 μg each of influenza B nucleoprotein DNA (B/NP)+blank DNA (total dose 200 μg/mouse on each occasion), three times at 2-week intervals. Two weeks after the last dose of DNA, mice were challenged with 100 mouse infectious dose (MID)50 of HK/486 intranasally. X31 virus-primed mice and their controls were challenged along with DNA-vaccinated mice. On day 6 after challenge, mice were sacrificed and lungs collected for titration of virus infectivity.
bDiffers significantly from A/NP+A/M group by analysis of variation (ANOVA), p=0.0082.
cDiffers significantly from A/NP+A/M group by ANOVA, p=0.011.
dDiffers significantly from unimmunized group by ANOVA, p<0.001.

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