Volume 8, Number 8—August 2002
Dispatch
Hep-2–Adherent Escherichia coli Strains Associated with Acute Infantile Diarrhea, São Paulo, Brazil
Isabel C. A. Scaletsky*
, Sandra H. Fabbricotti*, Sueli O. C. Silva*, Mauro B. Morais*, and Ulysses Fagundes-Neto*
, Sandra H. Fabbricotti*, Sueli O. C. Silva*, Mauro B. Morais*, and Ulysses Fagundes-Neto*Table 1
DNA probes identifying diarrheogenic Escherichia coli pathotypesa
| E. coli pathotype | Specific for | DNA probe description | Reference |
|---|---|---|---|
| ETEC | LT enterotoxin | pCVD403 (1.3-kb BamHI) | 13 |
| STp enterotoxin | pCVD426 (157-bp PstI) | ||
| STh enterotoxin | pCVD427 (216-bp EcoRI) | ||
| EIEC | Invasion | pPS55 (2.5-kb HindIII) | 14 |
| EHEC | Adherence | pCVD419 (3.4-kb HindIII) | 15 |
| Shiga toxin 1 | pJN37-19 (1.142-kb BamHI) | ||
| Shiga toxin 2 | pNN110-18 (842-bp SmaI-PstI) | ||
| EPEC | EAF plasmid | pJPN16 (1-kb BamHI-SalI) | 5 |
| eae gene | pCVD434 (1-kb SalI-KpnI) | 6 | |
| DAEC | daaC gene | pSLM852 (390-bp PstI) | 7 |
| AIDA-I | 8 | ||
| EAEC | AA plasmid | pCVD432 (1-kb EcoRI-PstI) | 12 |
aETEC, enterotoxigenic E. coli; EIEC, enteroinvasive E. coli; EHEC, enterohemorrhagic E. coli; EPEC, enteropathogenic E. coli; DAEC, diffusely adherent E. coli; EAEC, enteroaggregative E. coli; EAF, EPEC adherence factor; eae, encoding intimin, an outer membrane protein involved in the attaching and effacing lesions promoted by EPEC; daaC, associated with the biogenesis of F1845, a fimbrial adhesin involved in DA; AIDA-I, protein associated with the DA phenotype; AA, aggregative adherence plasmid.
