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Volume 9, Number 1—January 2003

Dispatch

Single Multiplex Polymerase Chain Reaction To Detect Diverse Loci Associated with Diarrheagenic Escherichia coli

Catalina López-Saucedo*, Jorge F. Cerna*, Nicolas Villegas-Sepulveda*, Rocío Thompson*, F. Raul Velazquez†, Javier Torres†, Phillip I. Tarr‡§, and Teresa Estrada-García*Comments to Author 
Author affiliations: *Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México D.F., México; †Hospital de Pediatría, México D.F., México; ‡Children’s Hospital and Regional Medical Center, Seattle, Washington, USA; §University of Washington School of Medicine, Seattle, Washington, USA

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Table 2

Diarrheagenic Escherichia coli isolates in patient and food samplesa

Samples Diarrheagenic E. coli group Identified genes No. positive strains/ no. tested Other pathogens
isolated CFU/gram
food
Stools





Patient 1
STEC
stx1,eae A
5/5
none

Patient 2
STEC
stx2
5/5
none

Patient 3
ETEC
lt
2/5
none

Patient 4
STEC
stx 2
2/9
none

Patient 5
STEC
stx 2
1/5
none

Patient 6
EIEC
ial
1/5
none

Patient 7
ETEC
lt
5/5
Shigella flexneri

Patient 8
ETEC
st
2/5
S. sonnei

Patient 9
EPEC
bfpA, eaeA
1/5
S. sonnei

Patient 10
ETEC
lt
1/5
Rotavirus, S. sonnei

Patient 11
STEC
stx1, eae A
1/10
Rotavirus

Food





Green sauce
ETEC
lt, st
5/5

8.0 X 102
Green sauce
ETEC
lt, st
5/5

1.3 X 105
Raw cabbage
STEC
stx1, stx2
2/5

2.6 X 105
Green sauce
ETEC
st
1/5

2.6 X 104
Green sauce
EIEC
ial
1/5

6.0 X 102
Raw coriander
EIEC
ial
1/5

1.8 X 105
Raw lettuce EIEC ial 1/5 8.2 X 104

aSTEC, Shiga-toxin–producing Escherichia coli; ETEC, enterotoxigenic E. coli; EIEC, enteroinvasive E. coli; EPEC, enteropathogenic E. coli.

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