Volume 9, Number 1—January 2003
Dispatch
Single Multiplex Polymerase Chain Reaction To Detect Diverse Loci Associated with Diarrheagenic Escherichia coli
Catalina López-Saucedo*, Jorge F. Cerna*, Nicolas Villegas-Sepulveda*, Rocío Thompson*, F. Raul Velazquez†, Javier Torres†, Phillip I. Tarr‡§, and Teresa Estrada-García*
Table 2
Diarrheagenic Escherichia coli isolates in patient and food samplesa
| Samples | Diarrheagenic E. coli group | Identified genes | No. positive strains/ no. tested | Other pathogens isolated | CFU/gram food |
|---|---|---|---|---|---|
| Stools |
|||||
| Patient 1 |
STEC |
stx1,eae A |
5/5 |
none |
|
| Patient 2 |
STEC |
stx2 |
5/5 |
none |
|
| Patient 3 |
ETEC |
lt |
2/5 |
none |
|
| Patient 4 |
STEC |
stx 2 |
2/9 |
none |
|
| Patient 5 |
STEC |
stx 2 |
1/5 |
none |
|
| Patient 6 |
EIEC |
ial |
1/5 |
none |
|
| Patient 7 |
ETEC |
lt |
5/5 |
Shigella flexneri |
|
| Patient 8 |
ETEC |
st |
2/5 |
S. sonnei |
|
| Patient 9 |
EPEC |
bfpA, eaeA |
1/5 |
S. sonnei |
|
| Patient 10 |
ETEC |
lt |
1/5 |
Rotavirus, S. sonnei |
|
| Patient 11 |
STEC |
stx1, eae A |
1/10 |
Rotavirus |
|
| Food |
|||||
| Green sauce |
ETEC |
lt, st |
5/5 |
8.0 X 102 |
|
| Green sauce |
ETEC |
lt, st |
5/5 |
1.3 X 105 |
|
| Raw cabbage |
STEC |
stx1, stx2 |
2/5 |
2.6 X 105 |
|
| Green sauce |
ETEC |
st |
1/5 |
2.6 X 104 |
|
| Green sauce |
EIEC |
ial |
1/5 |
6.0 X 102 |
|
| Raw coriander |
EIEC |
ial |
1/5 |
1.8 X 105 |
|
| Raw lettuce | EIEC | ial | 1/5 | 8.2 X 104 |
aSTEC, Shiga-toxin–producing Escherichia coli; ETEC, enterotoxigenic E. coli; EIEC, enteroinvasive E. coli; EPEC, enteropathogenic E. coli.
