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Volume 9, Number 12—December 2003

Dispatch

Noninvasive Method for Monitoring Pneumocystis carinii Pneumonia

Michael J. Linke*, Sandy Rebholz†, Margaret Collins†, Reiko Tanaka†, and Melanie T. Cushion*†Comments to Author 
Author affiliations: *Veterans Affairs Medical Center, Cincinnati, Ohio, USA; †University of Cincinnati College of Medicine, Cincinnati, Ohio, USA

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Figure

Progression of Pneumocystis carinii pneumonia measured by enumeration of organisms and real-time PCR of DNA extracted from lung homogenates and oral swabs. Panel A: Log P. carinii nuclei per mL of homogenized rat lung assessed by microscopic enumeration of lung homogenates; 4 wk vs. 7 wk, p < 0.001; 4 wk vs. 10 wk, p < 0.001; 7 wk vs. 10 wk, p < 0.001. Panel B: Log-transformed copies of P. carinii-specific DNA (mtLSU) per mL of lung homogenate; 4 wk vs. 7 wk, p < 0.001; 4 wk vs. 10 w

Figure. Progression of Pneumocystis carinii pneumonia measured by enumeration of organisms and real-time PCR of DNA extracted from lung homogenates and oral swabs. Panel A: Log P. carinii nuclei per mL of homogenized rat lung assessed by microscopic enumeration of lung homogenates; 4 wk vs. 7 wk, p < 0.001; 4 wk vs. 10 wk, p < 0.001; 7 wk vs. 10 wk, p < 0.001. Panel B: Log-transformed copies of P. carinii-specific DNA (mtLSU) per mL of lung homogenate; 4 wk vs. 7 wk, p < 0.001; 4 wk vs. 10 wk, p < 0.001; 7 wk vs. 10 wk, p > 0.05. Panel C: Copies of P. carinii-specific DNA/mL in oral swabs taken between immunosuppression (4, 7, and 10 wk preimmunosuppression) and at the time of euthanasia (4, 7, and 10 wk postimmunosuppression); pre- and post-4 wk, p = 0.0006; pre- and post-7 wk, p = 0.0037; pre- and post-10 wk, p = 0.0221. The ranges in value for the preimmunosuppression oral swabs at 4, 7, and 10 wk were, respectively, 165–487 copies/mL; 98–816 copies/mL; and 380–1,667 copies/mL. Ranges for the postimmunosuppression copies/mL after 4, 7, and 10 wk were 342–1,254; 517–5,256; and 637–6,279. (Number of rats in the 4- and 7- wk postimmunosuppression group = 8; number of rats in the 10-wk group = 6.)

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